https://t.co/YKn0ER4z5v
POTATOMap is officially out today (even tho it says 2024-12-30)… long story 😂
Constructs are available on addgene and POTATOMap data is available on PRIDE repository.
Thank you so much to everyone involved!!
It's out!😭🕺First preprint as a PhD at the Farias Lab @ginny_farias where we investigated biosynthetic lysosomal membrane protein trafficking, delivery and interactome with Protein Origin, Trafficking And Targeting to Organelle Mapping (POTATOMap)🥔
https://t.co/vmiYfjIx7F
Check out the preprint from Noortje investigating how polarised trafficking of neuroligin works in neurons. #POTATOMap_strikes_again~ Congrats to Noortje and everyone involved!
The main project of my PhD: "Spatiotemporal proteomics elucidates trafficking and localization of Neuroligins to the axon initial segment plasma membrane" is now on BioRxiv! Thanks to all collaborators🤝https://t.co/9ujbH3gzqF
Our manuscript on the role of the calsyntenin adaptors in biosynthetic axonal transport is on BioRxiv now: https://t.co/mKNr2KpemU
It was a pleasure to work on this with my colleague and friend @DerkDraper . Special thanks to @JKlumperman and @ginny_farias for their support.
Membrane editing with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles
https://t.co/kCOZ8NWfB5
Excited to share our preprint, now available online on BioRxiv! https://t.co/LjayTn0d6b. We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
To anyone who were excited for the release of SUM-PAINT, we now published a detailed protocol on to how replicate it in your hands! Check it out here: https://t.co/Wm8V3KuOhy
Thanks to @EduardUnterauer@EvaSchentarra@JungmannLab@euforna
Do you LOVE organelles??
Well I am THRILLED to announce that Nellie has been published in @naturemethods!
Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy
🧵1/N
ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬
The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime#microscopy
https://t.co/ukJcD7aZsE
Sanzà, Klumperman et al. @UMCUtrecht reveal a novel role of VPS41 in cellular trafficking, showing that it recruits biosynthetic LAMP carriers in a process dependent on the small GTPase Arl8b. https://t.co/3iyg6XcN77
#Organelles#Trafficking
Korsten, Kapitein et al. @UniUtrecht employ live cell imaging and expansion microscopy to show that nuclear polyQ aggregates associated with #HuntingtonDisease induce nuclear envelope (NE) blebbing and cause NE ruptures. https://t.co/xHcReT6jPp
#Disease#Membrane
Excited to share our new @Nature article reporting a novel cell polarity mechanism, mediated by contacts between the endoplasmic reticulum (ER) and plasma membrane (PM). Article: https://t.co/VRf5QIVkhG
Yesss!! It's out in @Dev_Cell!🥳Check it out to see lots of new exciting data on the axonal ER and local translation compared to the pre-print. Thanks to the reviewers for their insights that helped improve this study. And many thanks to all involved, a real team effort!
@AgChaigne@ginny_farias We are in the Netherlands, the land of potatoes 🥔🫢🇳🇱 Fun fact did you know Rabobank also has a potato map as well about world potato import/export?
https://t.co/r15GSnUNEo