Ashley T. Wong

Ph.D. Candidate @KomorLab | NIH NRSA Predoctoral Fellow @NIGMS | bioe | functional cancer genomics & precision genome editing

[Michael Love @mikelove]

Got an RNA-seq dataset with 50, 100, 200+ samples? Plug it into a differential expression tool and hope for the best? No! You need to consider QC, EDA, and modeling technical variation, or else risk generating spurious results. A thread on papers, methods, and best practices:

[Komor Lab @KomorLab]

Congrats to @kartik_lakshmi and @brodieranzau, whose paper analyzing RNA editing activity by adenine base editors is now out in @CRISPRjournal!
https://t.co/X6BGnK13Qk

[UCSD PATHS Program @PATHSUCSD]

Are you interested in pursuing a STEM degree at UC San Diego? Applications to join the next cohort of PATHS Scholars opens on March 1st and close April 15th! Share this amazing opportunity with graduating high school seniors who have applied to UCSD in a STEM major!🔬 #UCSanDiego

[Rahul Satija @satijalab]

Interested in single cell genomics but need help getting started? Check out the full agenda for our Single Cell Genomics Day next Friday (3/4). All talks will be live-streamed (no registration required) at https://t.co/vKIvFdsWzs

[Nature Reviews Cancer @NatureRevCancer]

NEW content online! CRISPR in cancer biology and therapy https://t.co/jznrosrQWH

[Ashley T. Wong @ashleytwong]

@patriciaclaudi0 Happy to chat with you about my app anytime you want!! Just send me an email :)

[Kartik Lakshmi Rallapalli @kartik_lakshmi]

Real team effort leads to awesome team logo. Thanks @ElizabethMPorto @ashleytwong @GuSifeng Mallory Evanoff and everyone else from @KomorLab!

[Nature Biotechnology @NatureBiotech]

Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs https://t.co/bQITDoZdvA

[Dow Lab @dow_lab]

New paper day! Since the development of #baseediting tools, we have been excited about the ability of this technology to engineer and understand the hundreds of cancer-associated genetic variants of unknown significance. BUT… it wasn’t as easy as hoped. 🧵https://t.co/An91ipVASJ

[Ashley T. Wong @ashleytwong]

Wordle 231 2/6

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Feels gooooooood

[Ashley T. Wong @ashleytwong]

Wordle 222 3/6 — I finally understand what these colored blocks all over my feed mean!!

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[Ashley T. Wong @ashleytwong]

@JohnDoench Wordle 225 4/6

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On my winning try I was like… these must be the only letters that make sense here?? I think this is a word??!

[Ensembl @ensembl]

Did you know that the Variant Effect Predictor (VEP) can tag 'stop gained' variants that are likely to escape nonsense-mediated decay (NMD)?

➡️ Read more in our blog:
https://t.co/dhCopoiLgb

#genomics #bioinformatics #VEP 🧬

[Ashley T. Wong @ashleytwong]

Had the privilege of attending my first in-person conference since beginning grad school! Huge thank you to all the organizers–including Alexis @KomorLab and Reuben @HarrisLabUMN for hosting #Deaminet 2022 safely. It was great to meet everyone!

[Quinn Cowan, PhD @Quinn_Cowan_sci]

Had an amazing time at the #Deaminet2022! Big thank you to Alexis @KomorLab, Reuben @HarrisLabUMN, and all the organizers for making it possible

[Illumina @illumina]

🟪⬜️⬜️⬜️🟪
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⬜️🟪⬜️🟪⬜️
⬜️⬜️🟪⬜️⬜️
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Not a wordle, just a chromosome.

[Kevin Holden @KevinHoldenSF]

It's been fantastic to be back at an in-person scientific conference! #Deaminet has been a brilliant conference with exciting new science in beautiful Palm Springs 🌴☀️😎 @Synthego is proud to have sponsored and supported this important meeting - Great to see everyone again! 🤓

[Ashley T. Wong @ashleytwong]

We routinely generate our own plasmids and would be SUPER interested in a dropbox program!! Thanks @JohnDoench for the (un-sponsored) rec!

[plasmidsaurus @plasmidsaurus]

4. Our dropbox program is expanding! Get in touch to set up a dropbox at your institution for free shipping!
(5/6)

[Florian Krammer @florian_krammer]

1) On January 25th 2023 a new data sharing policy from NIH kicks in which basically requires raw data from NIH-funded studies to be shared. Data is defined as:

[Joana Ferreira da Silva @FerreiraSilvaJ]

Check out this fantastic work summarising what we know about the repair of different CRISPR lesions. A must read!