Beamline I23 - Diamond Light Source

A unique instrument for in-vacuum long-wavelength macromolecular crystallography. Operating in the wavelength range 1.1Å - 5.9Å (11 keV - 2.1 keV).

[OxfordEnvRes @OxfordEnvRes]

@davidmpyle @OxfordGradStudy @PhDs_For_EDI @BlackInPlantSci @NCEOscience @UKRI_News @NERCscience @oxfordenvsust @ReedEcolNet @blackgeogorg #EnvironmentalScience #LifeScience #GoingToOxford #ActionNotWords

[Beamline I23 - Diamond Light Source @BeamlineI23]

AnACor2.0, the code for analytical absorption corrections now GPU-accelerated. Second paper of our fruitful collaboration with #OxfordEngineering.

[Journal of Applied Crystallography @JApplCryst]

Yishun Lu et al.: AnACor2.0: a GPU-accelerated open-source software package for analytical absorption corrections in X-ray crystallography #AbsorptionCorrection #RayTracing #LongWavelengthCrystallography ... #IUCr https://t.co/nDsOae6A7U

[Izzy Elliott @Izzy_G_Elliott]

Great to have the opportunity to present today at the @BioExcelCoE conference in Brno! @CCI_UoS @sotonbiosci

[Sam Horrell 🗿 @DrHorrell]

Successfully entered the world of Science Comedy last week.

This better not awaken anything in me (it definitely has).

[Beamline I23 - Diamond Light Source @BeamlineI23]

Metal ions play key roles in the catalytic activity of DNA-regulating enzymes like topoisomerases. Recent advances on I23 allow confirmation of Mg2+ and identify K+ and Cl- ions in bacterial topo IV complexes, extending our understanding of their function.

[Beamline I23 - Diamond Light Source @BeamlineI23]

Published yesterday in @PNASNews, a new paper from researchers at @imperialcollege, @ucl and @DiamondLightSou. I23 was used to identify Mg sites in the complex by collecting data below the S edge (2400eV - 5.17Å) on laser shaped crystals. https://t.co/39KflS5Lzb

[Beamline I23 - Diamond Light Source @BeamlineI23]

To confirm riluzole’s presence, SAD data were collected, identifying a riluzole sulphur atom with ∼20% occupancy. No such peak was found in DMSO-only crystals. This suggests that riluzole binds to the fenestrations. #Crystallography #NavMs #Riluzole #StructuralBiology

[Beamline I23 - Diamond Light Source @BeamlineI23]

Hot off the press! "Structural basis for the rescue of hyperexcitable cells by the amyotrophic lateral sclerosis drug Riluzole" used I23 data to pinpoint S atoms in a drug molecule. https://t.co/Ke86nkUY8c

[Beamline I23 - Diamond Light Source @BeamlineI23]

NavMs in the presence of DMSO, with and without riluzole, produced high-resolution crystals. While most electron density maps were similar, riluzole co-crystals revealed differences in the fenestrations, suggesting partial occupancy of both the detergent Hega-10 and riluzole.

[Beamline I23 - Diamond Light Source @BeamlineI23]

@ActaCrystD @IsmayForsyth Emerging tools like AlphaFill aim to improve ion predictions, highlighting the ongoing importance of experimental validation alongside machine learning models. #AlphaFold2 #StructuralBiology #ProteinResearch #ScienceTech

[Beamline I23 - Diamond Light Source @BeamlineI23]

Our latest paper is out in @ActaCrystD featuring work performed by our masters student @IsmayForsyth on f'' refinement for element identification. https://t.co/HKvH6vpJS7

[Beamline I23 - Diamond Light Source @BeamlineI23]

@ActaCrystD @IsmayForsyth However, discrepancies with experimental data exist in some high-confidence predictions. It’s crucial to validate AF2 models with experimental methods, including anomalous scattering, which can identify elements like metal ions.

[Beamline I23 - Diamond Light Source @BeamlineI23]

@ActaCrystD @IsmayForsyth AlphaFold2 has revolutionised protein structure prediction. Structural biology has quickly embraced its use, streamlining tasks like design, interaction prediction, and MR.

[Beamline I23 - Diamond Light Source @BeamlineI23]

In @eLife: Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling https://t.co/IMknLOxEWj

[Beamline I23 - Diamond Light Source @BeamlineI23]

High-confidence placement of low-occupancy fragments into electron density using the anomalous signal of sulfur and halogen atoms https://t.co/M9ZmtlDo7k

[Nature Communications @NatureComms]

Authors from @TheAsheLab, @BaldockLab including @t_malinauskas and @Gareth_R_Moore report structures of TWSG1 in complex with a BMP ligand and show how TWSG1 inhibits signaling.

https://t.co/ZWgPPkWBKd

[Beamline I23 - Diamond Light Source @BeamlineI23]

Structure and function of Semaphorin-5A glycosaminoglycan interactions https://t.co/0TeS4QKdGz
It was great to contribute to this paper from Yvonne Jones and team!

[Beamline I23 - Diamond Light Source @BeamlineI23]

Together with Wes Armour's group at @UniofOxford we developed a technique for analytical absorption corrections for crystallography. A very nice collaboration between @DiamondLightSou @UniofOxford @RCatHarwell @imperialcollege @RosFrankInst

[Journal of Applied Crystallography @JApplCryst]

Yishun Lu et al.: Ray-tracing analytical absorption correction for X-ray crystallography based on tomographic reconstructions #AbsorptionCorrection #RayTracing #LongWavelengthCrystallography ... #IUCr https://t.co/KKHoHHipYb

[Beamline I23 - Diamond Light Source @BeamlineI23]

Oligomerization mediated by the D2 domain of DTX3L is critical for DTX3L‐PARP9 reading function of mono‐ADP‐ribosylated androgen receptor - Vela‐Rodríguez - 2024 - Protein Science - Wiley Online Library https://t.co/41Xit99IOl

[Structure @Structure_CP]

Check out our new March issue:
https://t.co/j5KJUVfm2s

Our cover was designed by Kamel El Omari @Diamond_MX @RCatHarwell