Our in-situ structural analysis of the Ebola virus nucleocapsid is now published in @CellCellPress! Using cryo-FIB-ET, we revealed a 9Å structure, identifying novel layers and conserved interfaces that may serve as antiviral targets. #Ebola#Virology#StructuralBiology
Happy to share our new preprint: We imaged protrusions of Drosophila cells with cryoET and found filaments in the microtubule lumen. Subtomo-avg and RNAi suggest these are cofilin-bound actin (cofilactin). By @CamillaVenturaS in collab with Stephen Rogers.
https://t.co/0RFaY19Dph
So excited to share latest work from @Elliott_Lab_Ox! We find BIRC6, an enormous #ubiquitin IAP ligase, directly inhibits caspases and we show how this is blocked by SMAC. https://t.co/xSGWSKk1uh Plus great mass spec collab with @adanpinto@kesslerlabox
Check out our latest story! 🧬❄️🔬
https://t.co/4sJjI8NTUo
The structural connection between the linear nucleosome spacing, 3D tetranucleosome arrangement and linker histone occupancy! @mdombro_ @Sveta_Dodonova@c_dienemann @CramerLab
@nuno_5M @TBharat_lab @lab_chai @ProfSyK @ConradoPedebos @danielabbas99 Thanks! Indeed, we don’t think these are TasA, but rather a contaminant that becomes more pronounced due to the absence of TasA fibres. We sometimes see this contaminant in small amounts in wildtype samples as well.
Glad that I was able to contribute some EM to this exciting proof of concept: proteins can be softly deposited on grids using preparative MS and imaged in cryo. Interestingly, grids can be plunged into liquid N2 since they are not covered by a water film! Congrats to Tim and team