Thiagarajah Lab

Thiagarajah Lab Twitter @bostonchildrens, @harvardmed ac

[Thiagarajah Lab @JRTlab]

🧬 New preprint!
First data from the Gut-AGE study: A comprehensive cellular & spatial atlas of the pediatric duodenum integrating single-cell and spatial profiling with diet, social drivers of health, and environmental exposures.

https://t.co/w6J3ibPpUm

[Thiagarajah Lab @JRTlab]

@DrRosenIBD Thanks!

[Thiagarajah Lab @JRTlab]

@Lo_Zanzi Thanks Ivan!

[Thiagarajah Lab @JRTlab]

🚨 New lab paper ! 🚨
“A niche-dependent redox rheostat regulates epithelial stem cell fate.”
An incredible amount of work spearheaded by a very talented scientist - Xi Chen!
https://t.co/SHuPvb5XTW

[Thiagarajah Lab @JRTlab]

@LizaKonnikova Thanks Liza!

[Thiagarajah Lab @JRTlab]

@Synth_Immune See here! https://t.co/SHuPvb6vJu

[Thiagarajah Lab @JRTlab]

Intestinal stem cells live in niches that vary dramatically along the gut. But how do these microenvironments translate into changes in stem cell behavior?
Our new work shows that cellular redox balance is a key integrator. 🧬⚡

[Thiagarajah Lab @JRTlab]

Huge thanks to our collaborators and all the lab members who made this work possible! 🙌

[Thiagarajah Lab @JRTlab]

Mechanistically, redox state creates a self-reinforcing circuit linking HIF1α signaling to post-translational regulation of IDH1, a key metabolic enzyme.
This circuit couples local oxygen status to ISC metabolic programming. 🔄🧪

[Thiagarajah Lab @JRTlab]

By tuning cellular redox balance, the niche can shift the balance between asymmetric ISC self-renewal and lineage commitment suggesting that redox is a decision-making signal for stem cells. 🌱⚖️

[Thiagarajah Lab @JRTlab]

We found that hypoxia + Wnt signaling collaborate to restrict NOX1—a ROS-generating enzyme—to the crypt base in the distal colon.
This spatial control shapes where stem cells maintain an oxidative state needed for cell-cycle entry. 🔥

[Thiagarajah Lab @JRTlab]

We look at how intestinal stem cells integrate oxygen levels, Wnt signals, and metabolism for fate decisions in the unique environment of the distal colon

[Thiagarajah Lab @JRTlab]

Also Michael’s paper on an optimized end-to-end system and method for multiplex immunofluorescence imaging - check it out : https://t.co/rx8qZKu4S2

[Thiagarajah Lab @JRTlab]

A few lab updates: Our major consortium paper on the genetics of Congenital Diarrheas and Enteropathies came out last month. A major collab between BCH, SickKids (@AleixoMMuise), UCLA (Martin Martin) and Vanderbilt (@GoldenringLab ) https://t.co/JJJEl7wQMz

[Sahin Naqvi @snaqvi1990]

We are recruiting postdoctoral fellows!

Interested in using quantitative tools to study gene regulation in stem cell-derived developmental models? See below for more info and reach out

[Xu Zhou (周旭) @XuZhouLab]

Super thrilled to share a preprint from the lab https://t.co/LVXfNPgzVu
We found pH regulates the inflammatory response 🔥in #macrophage by controlling BRD4-dependent transcriptional condensates. In my lab led by @younggouu

[Journal of Cell Biology @JCellBiol]

Modeling the cell biology of monogenetic intestinal epithelial disorders. New Review from Izumi Kaji (@iKajiLab), Jay Thiagarajah (@JRTlab), and James Goldenring (@GoldenringLab) @VUMChealth: https://t.co/WRmiIBHbMa

#Disease #Physiology #Trafficking

[Ke Yuan @KeYuan_BCH]

New postdoc opening in my lab in vascular remodeling studies. We do cool science. Email if interested. @atscommunity @atsearlycareer @endothelialnews @ATS_PC @vascularbiology @jfftweets @UMassVascSurg @PVRI

[Charlie Evavold, find me @synth-immune.bsky.social @Synth_Immune]

Pleasant tidings that our paper's recognized as one of @CellReports Best of 2023.

Special congrats to @pascal_devant, @ElsyNgwa, & @BorsicElvira.

Great collaborators @HaopengXiao & @echouchani.

Super proud together with @JRTlab, @HafnerIva, & @jkagan1.

https://t.co/Wm45c0cxn8

[Thiagarajah Lab @JRTlab]

@pascal_devant @jkagan1 @WuLabHarvard @Nature @harvardmed @BostonChildrens @VirologyHarvard @HarvardMicro Congrats Pascal - great study