𧬠New preprint!
First data from the Gut-AGE study: A comprehensive cellular & spatial atlas of the pediatric duodenum integrating single-cell and spatial profiling with diet, social drivers of health, and environmental exposures.
https://t.co/w6J3ibPpUm
π¨ New lab paper ! π¨
βA niche-dependent redox rheostat regulates epithelial stem cell fate.β
An incredible amount of work spearheaded by a very talented scientist - Xi Chen!
https://t.co/SHuPvb5XTW
Intestinal stem cells live in niches that vary dramatically along the gut. But how do these microenvironments translate into changes in stem cell behavior?
Our new work shows that cellular redox balance is a key integrator. π§¬β‘
Mechanistically, redox state creates a self-reinforcing circuit linking HIF1Ξ± signaling to post-translational regulation of IDH1, a key metabolic enzyme.
This circuit couples local oxygen status to ISC metabolic programming. ππ§ͺ
By tuning cellular redox balance, the niche can shift the balance between asymmetric ISC self-renewal and lineage commitment suggesting that redox is a decision-making signal for stem cells. π±βοΈ
We found that hypoxia + Wnt signaling collaborate to restrict NOX1βa ROS-generating enzymeβto the crypt base in the distal colon.
This spatial control shapes where stem cells maintain an oxidative state needed for cell-cycle entry. π₯
We look at how intestinal stem cells integrate oxygen levels, Wnt signals, and metabolism for fate decisions in the unique environment of the distal colon
A few lab updates: Our major consortium paper on the genetics of Congenital Diarrheas and Enteropathies came out last month. A major collab between BCH, SickKids (@AleixoMMuise), UCLA (Martin Martin) and Vanderbilt (@GoldenringLab ) https://t.co/JJJEl7wQMz
We are recruiting postdoctoral fellows!
Interested in using quantitative tools to study gene regulation in stem cell-derived developmental models? See below for more info and reach out
Super thrilled to share a preprint from the lab https://t.co/LVXfNPgzVu
We found pH regulates the inflammatory response π₯in #macrophage by controlling BRD4-dependent transcriptional condensates. In my lab led by @younggouu