Here’s a review of DNA extraction methods developed for point-of-need DNA-based testing that could be useful for DNA testing practitioners, students of PCR, fieldwork researchers, and anyone interested in DNA extraction chemistry.
The authors, Lee et al. (2023), review methods suitable for use outside of conventional laboratories, ranging from quick and dirty extractions to affordable and easy-to-use extraction testing devices for mass distribution, such as lab-on-a-chip devices.
They also dive into the chemistry and processes behind each extraction option with referenced examples, comprehensive tables, and some great illustrations of the different methods. So this could be useful even if all of your work is lab-based.
Importantly, the authors examine recent DNA extraction methods devices in the context of the World Health Organisation’s REASSURED criteria for point-of-need testing, and assess their usefulness for users within this framework.
You can read the article here:
Lee et al. (2023). Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review. Biosensors, 13(11), 980.
https://t.co/5gyoj3ndzn
Unfortunately, the authors didn't cover filter paper-based DNA extraction methods, which is a super-cheap and easy method which has been suggested as meeting numerous REASSURED criteria. You can find an example of this method below:
Aula et al. (2023). Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples. Infectious Diseases of Poverty, 12(1), 71.
https://t.co/4QOVUOgdsL
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https://t.co/5gyoj3ndzn
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