Two complementary studies from our group out today: a therapeutic peptide vaccine trial for fibrolamellar carcinoma (FLC) & TIRTL-Seq, our robust method for deep quantitative paired TCR sequencing a🧵w/ @MarkYarchoan & @pogorely https://t.co/BN0pZK1JT0 & https://t.co/zzVmLA5zuo
Thanks to Caitlin Mills and a team of outstanding scientists the DyeDrop method together with a great demonstration of its use has been published. Who knew that playing with Nycodenz and some dyes 10 years ago would ultimately result in something useful. https://t.co/muNXgynEIE
New paper out at Nature Communications.
It details the Dye Drop assay for measuring numbers of live and dead cells and outlines a growth-rate correction method that disentangles cell death from arrest in drug-response assays
Dye Drop is a versatile, low-cost, and reproducible assay that uses sequential density displacement to obtain detailed cell cycle information in high throughput.
Want to try it? Visit https://t.co/KobpMR3zE5 to learn how!
@NatureComms https://t.co/nkRBnPWnGP
Proposal: PhD-level course in "how to get stuff to build and run". The final exam assigns you a random GitHub repo for a paper from no less than five years ago and asks you to get it running within 24 hours