Runze Mao

now Postdoc @francesarnold | PhD @HuLab_EPFL | @snsf_ch​ Postdoc | 2019 Reaxys PhD Prize finalist | Synthesis focused, #directedevolution #biocatalysis driven

[Runze Mao @runzemao]

@JuntaoYe @NatureCatalysis @francesarnold Thank you, Juntao!

[Runze Mao @runzemao]

Published today in @NatureCatalysis : https://t.co/szEvhUMf0z @francesarnold

[Runze Mao @runzemao]

Today we report in @ChemRxiv a biocatalytic strategy for enantioselective amination of 3° C–H bonds, providing chiral α-tertiary primary amines with up to >99% e.e., all in challenging intermolecular and notorious Me–Et substituted settings, w/o DGs https://t.co/01wkDRrnq9

[Runze Mao @runzemao]

For the 3rd time in a row, our work has been highlighted by Synfacts!
@thiemechemistry
https://t.co/mdWw6Dir56 Check out how we use engineered enzymes to build diverse lactone structures!
@francesarnold
#directedevolution #proteinengineering #enzymes #biocatalysis

[Runze Mao @runzemao]

New Year, New Paper🚀Today in @J_A_C_S, we report a biocatalytic platform that employs a C–C bond disconnection strategy to broaden Nature's lactone synthesis approaches, which rely solely on a C–O bond disconnection. https://t.co/YNvLi1RWIR

[Runze Mao @runzemao]

Check out this cool work from our lab @francesarnold, and learn how to use engineered enzymes to break stubborn carbon-silicon bonds. #enzymes and #DirectedEvolution never cease to amaze!

[Frances Arnold @francesarnold]

We are thrilled to present a new #enzyme to the world, the first shown to break the man-made carbon-silicon bonds in #siloxanes, which are persistent in the environment.
https://t.co/BJOuWk4WGW

[Frances Arnold @francesarnold]

We are thrilled to present a new #enzyme to the world, the first shown to break the man-made carbon-silicon bonds in #siloxanes, which are persistent in the environment.
https://t.co/BJOuWk4WGW

[Runze Mao @runzemao]

Recently, for the second time in a row, our work has been highlighted by Synfacts! @thiemechemistry https://t.co/2v5VWU2EVq
Check out our biocatalytic, stereoconvergent work! @francesarnold #directedevolution #proteinengineering #enzymes #biocatalysis

[Runze Mao @runzemao]

In @NatureSynthesis today, we report an engineered enzyme enabling #Stereoconvergent alkylation of alkene mixtures, which diverges from typical #stereospecific biotransformations, adding a fresh dimension to biocatalytic alkene functionalization. https://t.co/a1IjrvJ7dn

[Runze Mao @runzemao]

New year🎉New paper alert🚨 For a Research Briefing I wrote with @francesarnold of this work @NatureSynthesis, please access it via https://t.co/1jfrfCJeNU #directedevolution #proteinengineering #enzymes #biocatalysis

[Runze Mao @runzemao]

In @NatureSynthesis today, we report an engineered enzyme enabling #Stereoconvergent alkylation of alkene mixtures, which diverges from typical #stereospecific biotransformations, adding a fresh dimension to biocatalytic alkene functionalization. https://t.co/a1IjrvJ7dn

[Frances Arnold @francesarnold]

Dan Wackelin and @runzemao have made some pretty neat lactones (and other molecules) using a new-to-nature #carbenetransferase #enzyme. #directedevolution #biocatalysis. Happy 2024!

[Runze Mao @runzemao]

@agr_chemistry @J_A_C_S Thank you!

[Runze Mao @runzemao]

New Year, New Paper🚀Today in @J_A_C_S, we report a biocatalytic platform that employs a C–C bond disconnection strategy to broaden Nature's lactone synthesis approaches, which rely solely on a C–O bond disconnection. https://t.co/YNvLi1RWIR

[Runze Mao @runzemao]

Great collaborations w Dan, @kaichen_1990, @kathleen_sic, Yutao, @Anuvab_tweet. Special thanks to my supervisor @francesarnold!! #directedevolution #proteinengineering #enzymes #biocatalysis

[Runze Mao @runzemao]

After 20+ rounds of #directedevolution, we obtained engineered enzymes that catalyze carbene insertions to make 5-, 6-, 7-, 8-membered, fused-, spiro-, and bridged-lactones, even unprotected lactam and cyclic ketone from simple SMs with up to 5600 total turnovers and >99% ee.

[Runze Mao @runzemao]

@ME_Moret @ChemRxiv Thank you, Marc!

[Runze Mao @runzemao]

Today we report in @ChemRxiv a biocatalytic strategy for enantioselective amination of 3° C–H bonds, providing chiral α-tertiary primary amines with up to >99% e.e., all in challenging intermolecular and notorious Me–Et substituted settings, w/o DGs https://t.co/01wkDRrnq9

[Frances Arnold @francesarnold]

Exciting work from @runzemao...#enzymes can work with selectivity and efficiency that other catalysts just can't reach. And this is not even a natural reaction! #biocatalysis #directedevolution

[Runze Mao @runzemao]

@SGonzalezGranda @ChemRxiv Thank you!

[Runze Mao @runzemao]

Great collaborations w @Shilong_Gao @ziyangnickqin @torben_rogge @houk1000 @sophiajwu02 @ZiqiLi0513 @Anuvab_tweet. Special thanks to my supervisor, @francesarnold !!! #directedevolution #proteinengineering #enzymes #biocatalysis

[Runze Mao @runzemao]

Context: Despite advancements in asymmetric functionalization toward 2° C–H bonds, introducing chirality to racemic 3° carbon centers remains elusive. Once again, in intermolecular settings and without the assistance of DGs. To better comprehend this challenge, see below

[Nature Synthesis @NatureSynthesis]

Now online:

Article by @runzemao, Doris Mia Taylor, Daniel J. Wackelin, @francesarnold and co-workers @CaltechCCE

Biocatalytic, stereoconvergent alkylation of (Z/E)-trisubstituted silyl enol ethers

https://t.co/rN2e2KifrW ($)

[Runze Mao @runzemao]

Thanks to @ListLaboratory for the 'traffic light' analogy inspiration. 🚦 https://t.co/4bptoJ3VfI #directedevolution#proteinengineering #enzymes #biocatalysis @CaltechCCE @uclachem @NatureSynthesis

[Runze Mao @runzemao]

In @NatureSynthesis today, we report an engineered enzyme enabling #Stereoconvergent alkylation of alkene mixtures, which diverges from typical #stereospecific biotransformations, adding a fresh dimension to biocatalytic alkene functionalization. https://t.co/a1IjrvJ7dn

[Runze Mao @runzemao]

Many thanks to co-first Doris Taylor and Dan Wackelin for an exceptional collaboration, MD simulations by @torben_rogge under guidance from @houk1000, experimental support from @sophiajwu02 @kathleen_sic Heartfelt thanks to my supervisor @francesarnold for invaluable guidance!