Reconstitution of bidirectional #mRNA motility with the help of kinesins and #microtubule dynamics! https://t.co/ilkHy4uTCJ Movie: KIF5A (magenta) drags APC-RNPs (cyan) along microtubules. Thanks to @SebasBaumann, @GrawenhoffJulia, @ElsaCRodrigues&the lab!
APC couples several #mRNAs to different #kinesin motor proteins, EB1 & shrinking #microtubule ends which enables bidirectional mRNA motility on dynamic microtubules (mRNA=yellow,EB1=cyan). Thanks to @SebasBaumann, @GrawenhoffJulia, @ElsaCRodrigues&the lab! https://t.co/HowvUfIb5m
This is one of my last contributions to the RNA transport and in vitro reconstitution fields. I hope this helps others in the future to build crazy transport complexes and analyse their awesome single molecule TIRF data. Special thanks to Julia for here amazing contribution!
Finally someone’s getting the truth out about neuronal mRNA transport machineries;) If you are curious check out this very well researched review article by my colleagues @ElsaCRodrigues and @GrawenhoffJulia
Our view on what's known and where to look at regarding mRNA transport complexes!
Lots of fun digging through the literature and summarizing it for you 😍 take a look please!
Mammalian Neuronal mRNA Transport Complexes: The Few Knowns and the Many Unknowns https://t.co/bLVUzpeVxh
We're excited to describe our research on how gene products are distributed in skeletal muscle! We began these studies with the goal of identifying distinct mRNA localization patterns in muscle, anticipating that mRNAs encoding sarcomeric genes might be locally translated
I am super happy and thrilled that @forskningsradet funded my FRIPRO research grant on macropinosome formation. I will be looking for postdocs soon - if you are interested in membrane dynamics and want to play with cool microscopes, get in touch! @CanCell_UiO@OusResearch
I've never been a fan of GFP tags in my reconstitution assays, but when looking at these beauties I might have to reconsider - full length APC-GFP transported by Kinesin on paclitaxel-stabilised microtubules - full frames (512 x 512 pixels) recorded with @surrey_lab TIRF-M
12 #MotorsInQuarantine sessions wrapped up, 24 great talks, 24 great Q&A sessions, thanks to all speakers, all attendees, my fabulous co-hosts @zwetza, Huong, @EsquiviasRD_pau, @ChenYouchao, and @J_Cell_Sci for sponsorship. See you back in September! https://t.co/cx4buOvUCV
My latest postdoc work finally posted on Biorxiv.
Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression. https://t.co/Ozn7FK4L1c
Really nice talks and beautiful images from Jia Wang @NIH and @SebasBaumann @CRGenomicain on our 10th #MotorsInQuarantine today! Looking forward to seeing you all next week!