Felix Sigmund

Project Group Leader at @TU_Muenchen @MIBE_TUM and @HelmholtzMunich. Formerly Visting Scholar/Postdoc in @AdamEzraCohen 's lab at @Harvard.

[Felix Sigmund @sigisupertramp]

Excited to see our paper featured on the cover of the December issue of @NatureBiotech! A big thanks to the editorial team for recognizing our work on genetically-encoded, multiplexable EM labels.

[Nature Biotechnology @NatureBiotech]

The December issue is live https://t.co/AB51a7HbAp

On our cover, Sigmund et al. combine heavy metal binders with proteins self-assembling into spherical shapes to generate six different concentric barcodes that are readable by standard EM techniques https://t.co/ADG9AJFp2z

[Felix Sigmund @sigisupertramp]

Thrilled to receive an #ERCStG for BIO-CACHE! We’re engineering cellular flight recorders to log transient biological events in living cells over time. 🙏 to
@ERC_Research, reviewers and panel members, mentors and students! Want to build cellular black boxes? DMs are open! 🧬✈️

[Felix Sigmund @sigisupertramp]

@_Viroscope Hi, what are the puncta imaged on what scope? :)

[Helmholtz Munich | @HelmholtzMunich @HelmholtzMunich]

Engineering Smart Delivery for Gene Editors 🧬
👉https://t.co/4kiYAqoIQT

A research team from #HelmholtzMunich and @TU_Muenchen has developed an advanced delivery system that transports gene editing tools based on the CRISPR/Cas9 gene-editing system into living cells with significantly greater efficiency than before.

💡 Their technology, ENVLPE, uses engineered non-infectious virus-like particles to precisely correct defective genes – demonstrated successfully in living mouse models that are blind due to a mutation.

🌟 Precisely corrects defective genes
🌟 Promising for cancer therapy & inherited diseases
🌟 Enables creation of universal, off-the-shelf immune cells

@Earthnut89
#GeneEditing #CRISPR #CancerTherapy #Biomedicine #TranslationalResearch

[Dong-Jiunn Jeffery Truong @Earthnut89]

https://t.co/8Cwm3nmUzS
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @CellCellPress. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models.
Congrats co-first-authors @geilinho & @niggles293T!

[Jana Kroll @kroll_jana]

Excited to share our new preprint "Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons" https://t.co/JiPta0kDGg

We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during vesicle fusion ❄️🔬⏱️

[Felix Sigmund @sigisupertramp]

@NVIDIAGeForce #GeForceRTX50

[Felix Sigmund @sigisupertramp]

@NVIDIAStudio #GeForceRTX50

[Felix Sigmund @sigisupertramp]

@NVIDIAGeForceDE #GeForceRTX50

[Anna Schroeder @anna_schroed]

Schroeder Lab website is now live! 🔥 Check it out 🤩 to learn about who we are, what we do to study how the brain gives rise to internal states that drive adaptive behavior 🧠, and how you can join the team 👇 Spread the word! https://t.co/K66Wp6MVXq

[Roman Jerala @rjerala]

In our new paper we extended modules for building protein polyhedral cages from coiled-coil (CC) dimers to tetrameric CCs. This introduced many new topological possibilities and allowed assembly of dimeric cages.
https://t.co/xtpYzKTawC 🧵

[Niklas Armbrust @niggles293T]

Excited to share 'non-destructive transcriptomics via vesicular export' (NTVE). We use HIV-1 virus-like particles, to export 'snapshots' of a cell’s transcriptome, which allows to follow gene expression changes over time by sampling from the supernatant. https://t.co/bOIQdHpxMB

[Dong-Jiunn Jeffery Truong @Earthnut89]

Check out our approach on "non-destructive transcriptomics via vesicular export" (NTVE)! We have benchmarked NTVE exclusively in stably integrated cell lines, where transcript alterations by perturbations were truthfully reflected in the supernatant. ,https://t.co/17BhLA9mJp

[Giessen Lab @GiessenLab]

New paper out at @acsnano. Awesome work by PhD student Seokmu Kwon (@smkwon6) and postdoc Mike Andreas! https://t.co/Kdi02jrFQy

[Giessen Lab @GiessenLab]

New paper out @NatureComms. Tremendous work by postdoc Mike Andreas, congrats! https://t.co/XyMj5lThtH

[Alice Ting @aliceyting]

APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2 is a liability. We now report LaccID, a multicopper oxidase evolved from an ancestral fungal laccase that oxidizes aromatic substrates using non-toxic O2 instead of H2O2. We use LaccID for EM imaging of cultured cells and fly brains, and for proteomic mapping of the changing surface composition of T cells that engage with tumor cells via antigen-specific T cell receptors.

[Vasilis Ntziachristos @ntziachristos]

So happy to announce the next Helmholtz Pioneer Campus (HPC) Principal Investigator (f/m/x) recruitment round to promote wonderful young talent in Bioengineering and Biomedicine. https://t.co/P2yRMYFYHR

[Vasilis Ntziachristos @ntziachristos]

Controlling the absorption of certain molecules imparts new opportunities for high-resolution #optoacoustic biological imaging, much deeper than what is possible by optical microscopy. We argue that such absorption-switchable labels may play for optoacoustic imaging the same role that fluorescent proteins play for optical imaging and microscopy.

[stiel_lab @stiel_lab]

Switch on ... Excited to share thoughts on switching proteins in Photo/Optoacoustics together with @ntziachristos in @naturemethods . Support for ongoing work from @erc_research #Switch2See , @MunichImaging , @HelmholtzMunich , @uni_regensburg

[Anne Carpenter, PhD @DrAnneCarpenter]

Wow, huge scale electrophysiological screen in iPSC neurons. 30,000 compounds screened to rescue a TSC2 loss of function phenotype (all optical!)

They found the phenotype w CRISPR & patient cell lines, in ephys, morphology, and mRNA.

(I’m on Quiver SAB)
https://t.co/N5kl5e2iwT

[EMBL Events @EMBLEvents]

🙋🏻🔬 #EMBOinsitu is open for registrations!

From cryo-EM to multi-scale modelling - what types of biological questions can be addressed using in situ structural biology?

📜 Submit abstract by 12 Nov

📅 4 – 7 Feb 2025
📍 EMBL Heidelberg and Virtual

➡️ https://t.co/zO2QG19gpX