Tulika Sharan

PhD candidate at UW Madison Raman Lab👩‍🔬 (she/her)
protein folding enthusiast

[Vatsan Raman @vatsanraman]

Viruses reprogram host cells by reshaping chromatin, but the epigenetic activities of most viral proteins remain unknown. In our new preprint, we use pooled single-cell epigenomic screening to profile 110 viral transcriptional regulators simultaneously, revealing widespread chromatin remodeling, unexpected functional convergence across diverse viruses, and shared targeting of NF-κB, AP-1, p53, and Sp1/KLF regulatory networks. Drug perturbation screens further uncover host pathways required for viral chromatin reprogramming. Phenomenal work by James Corban @james_corb85937!
https://t.co/J0HqSQ6keY

[Vatsan Raman @vatsanraman]

Phage metagenomes hold powerful secrets! This article breaks down our recent work using Meta-SIFT to uncover functional motifs hidden in viral genomes—and how we used them to engineer phages that kill bacteria responsible for food-borne outbreaks. @ScienceAdvances
Phenomenal work by @phil_huss & fun collab with @KarthikGeomicro.

[Vatsan Raman @vatsanraman]

Thrilled to unveil PhageMaP—a powerful method for mapping phage gene essentiality and decoding gene-environment-function relationships. We created comprehensive gene essentiality maps for model and non-model phages across diverse hosts. Discovered key 'counters' & 'triggers' to evade host defenses and engineered phages with enhanced infectivity. Big shoutout to Jackie Chen for his phenomenal work!
https://t.co/jVpVIXA5VG

[Vatsan Raman @vatsanraman]

Excited to share PROD-ATAC, a single-cell tool to study chromatin perturbations caused by protein-coding variants. PROD-ATAC revealed mechanisms and relationships among oncofusions in 30+ cancer types, generating a global view of the oncogenic landscape. Phenomenal work done by @CasualVariant
https://t.co/sOnTgteBMH

[UW–Madison Biochem @UWBiochem]

Today: Join @UWBiochem and @UWGlobalHealth for the first of two lectures by Tulio de Oliveira (@Tuliodna), director of @ceri_news, on tackling global health security threats. Learn more about this scientific lecture & get the livestream link: https://t.co/mp09udos2Z

@illumina

[Vatsan Raman @vatsanraman]

Excited to present PHAGEPACK for quantifying genome-wide which bacterial host factors impact phage infectivity. PHAGEPACK combines CRISPRi with phage packaging. Reveals phage-host interactions, evolution, & host resistance. Great work by @chuchitboonthav!
https://t.co/huGFOVcf39

[Vatsan Raman @vatsanraman]

How do we interpret molecular mechanisms of protein allostery from deep mut scan experiments? We have built a generalized thermodynamic model to infer and predict how mutations impact inter-and-intradomain allostery. Great collab with Qiang Cui BU
@eLife https://t.co/0YKJNaEc75

[Vatsan Raman @vatsanraman]

Our new study demonstrates extreme ruggedness and high epistasis in the evolutionary landscape of a TF family. Gain/loss function between adjacent nodes, rapid switches in specificity and spontaneous GoF at distinct clades. @Jackson_Lab @CellSystemsCP
https://t.co/IX0lmEJZQA

[Tulika Sharan @tsharan97]

Friday lab shenanigans @phil_huss

[Scott Coyle @CellRaiser_]

Our first paper is out in @CellCellPress! Led by @born2raisecell, we present a programmable reaction-diffusion system that generates synthetic protein waves, oscillations, and patterns for spatiotemporal circuit design and FM data-encoding in human cells. https://t.co/lNRtRdJAxx

[Dr Meming @Dr_Meming]

Me giving advice to new PhD students

[UW–Madison Biochem @UWBiochem]

For over 20 years, Professor James Ntambi has paved the way for students to gain their own understanding of metabolic disorders while connecting with communities throughout his native country of Uganda. Read more in our Q&A: https://t.co/FywsSBvg1D

[Dipa Sashital @dsashital]

I'm excited to share our findings on a metal-dependent specificity switch for #CRISPR-Cas12a! We profiled the specificity of Cas12a at four Mg2+ concentrations and observed striking differences in mismatch tolerance at lower, physiological conditions 1/n
https://t.co/BO33CYAttp

[Tulika Sharan @tsharan97]

From attending #RosettaCon this summer to participating in the Rosetta + ML bootcamp @ucdavis, I'm beyond thrilled to be a part of the @RosettaCommons community! Thank you for a great time! Excited to get protein engineering 🧬🖥✨️

[Max Frenkel, MD, PhD @CasualVariant]

Excited to share my thesis work: Discovering chromatin dysregulation induced by protein-coding perturbations at scale

Reading DNA is fast and high-throughput. Discovering mechanisms is slow and low-throughput. A 🧵 on how we addressed this 1/10
https://t.co/DkcxvOFS5Z

[UW-Madison IPiB @uwmadisonipib]

The IPiB community came together at last week's IPiB Retreat to hear about the latest in research from @UWBiochem and @BMC_UW labs. More photos from the day on Flickr: https://t.co/LwetTZk2F4. Thank you to #GLDC leaders for organizing!

[Aditi Sharan @AditiSharan15]

It was an absolute honour to present my work in front of you! What else can I say!🙏 Thank you so much @MF_GDG and @GAD_Aus for your kind words of support and motivation 😊😊😊
#ISAWCS23 @isa_sociology

[Dr Meming @Dr_Meming]

Eeep

[Tulika Sharan @tsharan97]

inspired by real events

[Tulika Sharan @tsharan97]

No google, I'm definitely looking for that other thing, how can you not know smh