Wei Du

Ph.D., University of Amsterdam, Microbial metabolic engineering, Cyanobacterial CO2 conversion

[Wei Du @WeiDu13]

@haihe10 @erblabs @mpi_marburg Thank you for the explanation! So this tigher auxotrophy is only for PN and cells seems just need more time to evolve (find a solution)... I am a big fan of tigher auxotrophy, but also amazed by how microbes to fix it. Again, great work!

[Wei Du @WeiDu13]

@haihe10 @erblabs @mpi_marburg Very interesting work! Two questions: 1. the suppressor mutants seems selected earlier with 13nM pyridoxine added compared with no pyridoxine, because of random evolution trajectories? 2. is the tighter auxotrophy due to both threonine (less added) and pyridoxine auxotrophy?

[Wei Du @WeiDu13]

@KSechkar Nice work! I like this concept...Hope to have experimental validation of this concept.

[Wei Du @WeiDu13]

@BruinsmaLyon @ChrisBatianis Curious to know how long the pyruvate-auxotrophy stays? We previously got the published (till 30 hour's cultivation) E.coli pyruvate-auxotrophic strain. But after 48 hours cultivation in our lab, it is not auxotrophic any more :-(

[Wei Du @WeiDu13]

@HuadongPeng @VictoriaHaritos @LedesmaAmaro @MiKrogh @apsduk @willmshaw just read a paper from you--A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities. Super interesting!

[Wei Du @WeiDu13]

@NicoC_MicSynBio Nice article and nice work, though I would still think Nature is much smarter;-) there is also much work we can do (has done) to improve the efficiency of calvin cycle especially in cyanobacteria for CO2 conversions.

[Wei Du @WeiDu13]

@frantecol Yes. Lucky to be the 10Kth one

[Wei Du @WeiDu13]

@Kylelauersen I think this bio-electrochemical hybrid could be useful for those cells to get more photons from electricity instead of light.

[Wei Du @WeiDu13]

@Kylelauersen Indeed, many photons are not used for the algae cells facing directly towards light. But for the ones hiding behind the light sources because of the cell shading or limited light penetration in a thick/dense culture, they would still need more photons.

[Wei Du @WeiDu13]

@johntheap @ProfTomEllis @NAR_Open @George_M_Taylor @HitchcockLab It would be also great to find a way to harmonize the cellular resources competition between cell growth and production formation. To me it seems this is one of the main problems of genetic unstable production. Anyway, glad to see this paper comes out ;-)

[Wei Du @WeiDu13]

@johntheap @ProfTomEllis @NAR_Open @George_M_Taylor @HitchcockLab Great work! Congratulations! To me why this approach can achieve a stable production is, this approach can maintain a perfect flux balance for this multi-step enzymatic reactions via the libraries-based optimization of each promoter/RBS, right?

[Wei Du @WeiDu13]

@Branco_d_Santos Good paper for literature discussion

[Wei Du @WeiDu13]

@SILS_UvA Haha, yes...BTW, we were the champion in 2017 as well...

[Nature Reviews Microbiology @NatureRevMicro]

The same immune systems that protect hosts from infection can ratchet up the evolution of more-harmful pathogens @sciencemagazine https://t.co/tJdHO7YsmC