Will Shaw

Postdoc in Mo Khalil’s group at Boston University

[Thomas Gorochowski @chofski]

Come and join us at a free complementary Japan-UK Engineering Biology meeting after the main ESSL conference (https://t.co/fFiRTs6GnS). A few spaces left to some see some of the up and coming superstars of #synbio in the UK!

[Jonas Koeppel @JonasKoeppel]

Genome engineering excelled at small nucleotide changes, but large edits remained challenging. Recent innovations are changing this.

We reviewed the field of structural variant engineering @NatureGenet 🧬

@JulianeWeller @TVanderstichele @LeopoldParts

https://t.co/vwEEqHQFZC

[Sarah Guiziou @sarah_guiziou]

Our paper: "A history-dependent integrase recorder of plant gene expression with single-cell resolution" is published in @NatureComms . Congrats Cassandra Maranas for completing this work! @EarlhamInst @UWBiology
To read the paper: https://t.co/l8TWW2TQhb
https://t.co/fbphXGdDcB

[Sarah Guiziou @sarah_guiziou]

Job alert: 2 postdoc positions available in my group @EarlhamInst. Synthetic biology to 1) decode and engineer root architecture, 2) engineer plant-beneficial rhizobacteria. Reach out and please share!
https://t.co/kQQZaegZK6
1) https://t.co/kWkXnFFPo7
2) https://t.co/7CE3Ozhu9q

[Fankang Meng @DrFankangMeng]

Excited to share our latest research on "Engineered Yeast Multicellularity via Synthetic Cell-Cell Adhesion & Direct-Contact Signalling" posted on @bioRxiv #synbio recently! 🧵🔗[DOI](https://t.co/7F0FXCrJmw)

[Patrick Hsu @pdhsu]

What if we could universally recombine, insert, delete, or invert any two pieces of DNA?

In back-to-back @Nature papers, we report the discovery of bridge RNAs and 3 atomic structures of the first natural RNA-guided recombinase - a new mechanism for programmable genome design

[ElowitzLab @ElowitzLab]

@arjunrajlab @markwbudde @UriAlonWeizmann In case it's helpful to anyone, I posted the evolving set of talk tips that I share with my lab on our website. Scroll to bottom of this page: https://t.co/ktvRtvp61G.
(It was once "10 commandments" but has since expanded to 25...the slide titles are currently number 5)

[Caroline Dean @CarolineDeanLab]

Check out the movie that summarises our papers out now in @MolecularCell https://t.co/dgbwridV7H🧬🎞️

[Will Shaw @willmshaw]

@GallowayLabMIT @ProfTomEllis @chiara_masnovo Fig S11 https://t.co/6wWm8LphH3

[Will Shaw @willmshaw]

@GallowayLabMIT @ProfTomEllis @chiara_masnovo They all have unimodal behavior. The yeast were grown to saturation with inducer, back diluted in fresh media with inducer, and measured after 6 h in mid-log phase. So this is the sustained response to inducer concentrations. TFs/reporters are single copy, stably integrated.

[Will Shaw @willmshaw]

@ZymoSuperMan @Addgene @ProfTomEllis @MoKhalilLab Yep, the CRISPR-Cas9 system can be used for any kind of editing you want to do in yeast. Just design the donor DNA for your target and follow the instructions in the paper for cloning the gRNA into the CRISPR-Cas9 plasmid (pMYT095)

[Will Shaw @willmshaw]

Our new yeast toolkit is now available on @addgene! Huge thanks to the whole team at Addgene for the heroic effort getting this kit ready for distribution.
https://t.co/C8966qczmk

Below is a summary of the toolkit... [1/17]
@ProfTomEllis @MoKhalilLab

[Tom Ellis @ProfTomEllis]

It's finally out... Will Shaw's awesome YTK add-on toolkit for rapid parallel yeast genome engineering is published today in @ACSSynBio @willmshaw @MoKhalilLab > If you do yeast synthetic biology, you're going to need this game-changing kit from @Addgene https://t.co/hpAU42HNMP

[Tom Ellis @ProfTomEllis]

New preprint from our lab is up on @biorxivpreprint - a really awesome synthetic genomics paper that Xinyu Lu from the team has been brewing for the last few years. @Xinyu80256297 https://t.co/R3jK3cWM16

[Tom Ellis @ProfTomEllis]

We're hiring! We're taking another big leap in using synthetic biology to engineer materials - this time for sustainable food and textiles. In the next few weeks, we've up to 4 postdoc positions opening up. I'd love to hear from anyone interested in joining us this year. Pls RT🔁

[Alice Boo @alicebioboo]

🌱📡 We've engineered bacteria to 'talk' to plants for programmable microbe-to-plant communication, opening new doors for sustainable agriculture: https://t.co/1z2F6XvfYV @NatureComms

[Jonas Koeppel @JonasKoeppel]

What if we could inducibly create thousands of structural variants and ecDNAs in human cells and see which ones survive?

https://t.co/tbMsyfu8md

I’m excited to share our story on scrambling the human genome by combining prime editing, repetitive elements, and recombinases. A🧵

[Asimov Press @AsimovPress]

Welcome to Asimov Press, a new publishing venture that features writing about biology.

Our magazine and books will cover a broad range of topics, including biosecurity, vaccine development, and the long arc of progress in genetic engineering.

Learn more: https://t.co/uAPG9sQMaN

[Will Shaw @willmshaw]

Importantly, the toolkit if fully backward compatible with YTK, so anything you have previously made with YTK will work with MYT and vice versa. We hope this new toolkit will allow current and future YTK users to take their yeast engineering goals to the next level! [17/17]

[Will Shaw @willmshaw]

If you haven’t already, you should also get the Dueber YTK from Addgene https://t.co/2oAVx5Lsb3.

You will need this to get the most out of MYT. In particular, the fantastic library of well-characterized promoters and terminators [16/17]