Katie Galloway

Associate Prof @MITChemE ; mom^4 + wife; enjoys building cell-fate circuits, exploring dna topology, reprogramming the living world, and soccer; soli gloria deo

[Katie Galloway @GallowayLabMIT]

Could the folding of synthetic gene circuits in 3D shape how genes are expressed? Today @ScienceMagazine we report on the role of gene syntax in shaping feedback between transcriptional activity and genome folding for advanced circuit design🧵 (1/n)

[Katie Galloway @GallowayLabMIT]

@MoEbrahimkhani @MONASERT @SynCellJoshua @samira_kiani1 very cool, Mo! Congrats!

[Howard Salis @hsalis]

Massively parallel assays using oligopools, barcoding, & deep sequencing follows the same story.

Replaces 100s of microtiter plates (one assay per well) with 10000s of experiments run in one pooled assay.

Work your 🧠, not just more 🤖.

[Katie Galloway @GallowayLabMIT]

@NoahOlsman @threadreaderapp unroll

[Arjun Raj @arjunrajlab]

All cell types are wrong, but some are useful.

[Katie Galloway @GallowayLabMIT]

@arjunrajlab perfect

[Katie Galloway @GallowayLabMIT]

@yogeshgoyallab @CellRepMethods @DeonPloessl @LaurieBoyer1221 @A_BlanchAsensio @eboyden3 @MITChemE @MITdeptofBE @kochinstitute @broadinstitute It has been for us! Let us know how it works for you!

[Katie Galloway @GallowayLabMIT]

What if a single RNA transfection could program human stem cell fate or enable functional biosensing for more than a month?
Our new @CellRepMethods paper explores how self-amplifying RNA makes this possible, without genomic integration. 1/n :🧵

[Cell Reports Methods @CellRepMethods]

Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs https://t.co/sUVUMoohQK

[Katie Galloway @GallowayLabMIT]

@nzmyachie @CellRepMethods @DeonPloessl @LaurieBoyer1221 @A_BlanchAsensio @eboyden3 @MITChemE @MITdeptofBE @kochinstitute @broadinstitute Thanks, Nozumu!

[Katie Galloway @GallowayLabMIT]

Integrating synthetic biology to understand and engineer the heart, lung, blood, and sleep systems
"By integrating genome engineering, dynamic gene circuits, and high-dimensional biosensors, these advances support scalable, quantitative models of multicellular biology, expanding the need for systems-level models and integration. We highlight emerging systems such as tunable transcriptional regulators, synthetic organizers, and feedback circuits that bridge molecular control with functional outcomes." https://t.co/lXzYaBvahV

[Katie Galloway @GallowayLabMIT]

Last year, we spent a lot of time thinking about to engineer some of the most complex cellular systems: lung, heart, blood, and sleep systems! In case you missed it, you should check out some of that vision from @SynBioGaoLab @sahakris @LaurieBoyer1221 @BashorLab @yogeshgoyallab @genegirl007 @isaacbhilton @Pulin_Li @jasonhyang @echaikof and many more! Link below:

[Katie Galloway @GallowayLabMIT]

What if a single RNA transfection could program human stem cell fate or enable functional biosensing for more than a month?
Our new @CellRepMethods paper explores how self-amplifying RNA makes this possible, without genomic integration. 1/n :🧵

[Katie Galloway @GallowayLabMIT]

@threadreaderapp unroll

[Katie Galloway @GallowayLabMIT]

Want to know more? You can read the paper here: https://t.co/sKdfKnzaT2