Dingchang Lin

Asst. Prof @JohnsHopkins; Broadly interested in neurotechnology, genetically encoded tools, and materials science.

[Dingchang Lin @DingchangLin]

🚨 Today in @Nature, we report GEMINI—a genetically encoded intracellular memory device that writes cellular dynamics into tree-ring-like fluorescent patterns within cytoplasmic protein assemblies.[1/n]
https://t.co/eVchPCiK6f

[Dingchang Lin @DingchangLin]

@MichaelLinLab Amazing method! I am curious how the transfection efficiency and expression level compare to plasmid transfection.

[Sholto David @addictedtoigno1]

Earlier this week I posted an example of a fake western blot provided by ThermoFisher to demonstrate the validity of a p53 antibody. I considered it an amusing curiosity. In fact ThermoFisher has systematically manipulated antibody validation data. Short Thread... 🧵

[Ali Max Erturk @erturklab]

Today in @Nature, we report MouseMapper: foundation-model AI to map disease perturbations across the entire mouse body cell-by-cell.
In obesity, it revealed body-wide inflammation & unexpected facial nerve damage. 🧵👇🔉
https://t.co/BERf5GQ10Z led by @Dorie00 & @yingchen733

[Shunzhi Wang @wang_shunzhi]

A growing protein cage has no architect.

Each piece only responds to its local environment.

Yet somehow, thousands of pieces can come together to form a large, precise sphere.

Today in @Nature, two back-to-back papers from our team and @UWproteindesign show how de novo protein design can make this kind of self-assembly programmable.

The trick: we didn't design perfect symmetry.

We designed the rules for breaking it. [1/17]

https://t.co/zHeNBc1oFt
https://t.co/MNS1bS9r5O

[Sholto David @addictedtoigno1]

Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures", but their own internal data.

[Dingchang Lin @DingchangLin]

@JHUBME @kalhorlab @JohnsHopkins Congrats! 👏 👏@kalhorlab

[Dingchang Lin @DingchangLin]

@JiangYuanwen Congrats Yuanwen!

[Katie Galloway @GallowayLabMIT]

Could the folding of synthetic gene circuits in 3D shape how genes are expressed? Today @ScienceMagazine we report on the role of gene syntax in shaping feedback between transcriptional activity and genome folding for advanced circuit design🧵 (1/n)

[Shunzhi Wang @wang_shunzhi]

1/ Excited to share our new Review in @Nature:
“The past, present and future of de novo protein design.”
Here, we mainly focused on structure-guided protein design. The field is entering a new phase: now that we can design new proteins, what should we build next?

[Julia Bauman @JuliaBauman2]

The most beautiful molecular recorder 😍

GEMINI is a self-assembling protein system that records cellular events as physical layers, readable via imaging. Achieves down to 15 min resolution and works nicely in vivo!

From @DingchangLin's lab in @Nature

[Johns Hopkins Engineering @HopkinsEngineer]

Congratulations to the 4 Hopkins Engineers among the 449 scholars newly elected to the American Association for the Advancement of Science (@aaas): Jeff Coller & Michael Miller in @JHUBME, Kalina Hristova in @JHUMaterials, & Rajat Mittal in @jhumeche! https://t.co/rYzmuHlcDi

[Dingchang Lin @DingchangLin]

@HongNeuroTech Thank you Guosong!

[Dingchang Lin @DingchangLin]

We made this work Open Access! Please check out👉 https://t.co/1jR5feCeqr

[Dingchang Lin @DingchangLin]

🚨 Today in @Nature, we report GEMINI—a genetically encoded intracellular memory device that writes cellular dynamics into tree-ring-like fluorescent patterns within cytoplasmic protein assemblies.[1/n]
https://t.co/eVchPCiK6f

[Yulong Li Lab @yulonglilab]

Excited to share our new paper on GRAB_PGE2-1.0, the first genetically encoded fluorescent sensor for monitoring spatiotemporal prostaglandin E2 (PGE2) dynamics in vivo! It enables real-time imaging in cultured cells, acute brain slices, and living mice.

[Denis Wirtz @deniswirtz]

We have developed a new AI-based assay to rapidly identify new molecular targets to inhibit cancer cell migration and metastasis.

See more details here: https://t.co/U7nYtiabZY

[Dingchang Lin @DingchangLin]

@xiongyih @Nature Thank you Xiongyi!

[Dingchang Lin @DingchangLin]

🚨 Today in @Nature, we report GEMINI—a genetically encoded intracellular memory device that writes cellular dynamics into tree-ring-like fluorescent patterns within cytoplasmic protein assemblies.[1/n]
https://t.co/eVchPCiK6f

[Dingchang Lin @DingchangLin]

@N_Rajewsky Sorry we are unable to share more details about our ongoing research in this open channel. But we are willing to share offline if you are interested.

[Dingchang Lin @DingchangLin]

We are actively recruiting postdocs to push this technology forward! If you are interested please reach out!

[Dingchang Lin @DingchangLin]

🚨 Today in @Nature, we report GEMINI—a genetically encoded intracellular memory device that writes cellular dynamics into tree-ring-like fluorescent patterns within cytoplasmic protein assemblies.[1/n]
https://t.co/eVchPCiK6f

[Dingchang Lin @DingchangLin]

@N_Rajewsky e.g. we are currently using GEMINI to study signaling dynamics in tumors w/wo drug treatment

[Dingchang Lin @DingchangLin]

@N_Rajewsky Push the technology to broader applications (recording more different stuffs and being used beyond our lab) and also to advance fundamental understandings.

[Dingchang Lin @DingchangLin]

@pdhsu Thank you Patrick!