Jonathan Jeffet

PhD student @TelAvivUni, Physics, Optics, single-molecule, Epigenetics.

[Jonathan Jeffet @JJeffet]

Awesome work! Very elegant way to get all the info in a single frame. Love it!

[Lucien Weiss @LucienWeissLab]

Woohoo!
Localization in 4D (x, y, z, λ) at video framerates - "Vortex light field microscopy: 3D spectral single-molecule imaging with a twist"
Thanks, @BoyaZhang4, @koholleran!
@samofmidworld, C. Zhu, M.O. Lens,@LisaMariaN91,@Ruby_Peters_,@SteveTheChemist https://t.co/aWlIBLwkCd

[Yuval Ebenstein @hagenom]

Just up on bioRxiv: Long-Read Structural and Epigenetic Profiling of a Kidney Tumor-matched Sample with Nanopore Sequencing and Optical Genome Mapping https://t.co/8SXgeB7hL9

[Yoav Lahini @YLahini]

Our latest research, just published in @NaturePhysics: slow relaxations and logarithmic aging occur via self-organized avalanches of local instabilities. Splendid work by @Yaniv_Friedman and Dor Shohat @ComplexLab_TAU @TelAvivUni
https://t.co/hM0ilhCUTL
https://t.co/2MfNFvE4we

[Yuval Ebenstein @hagenom]

Check out our new single-molecule chromatin profiling method for ultralong DNA molecules with @bionanogenomics : Dam Assisted Fluorescent Tagging of Chromatin Accessibility (DAFCA) for Optical Genome Mapping in Nanochannel Arrays. https://t.co/y94JeE9K7B Please RT

[Yuval Ebenstein @hagenom]

I’m not sure 🏝CoCoS microscopy 🏝 got the visibility it deserved but it’s the most read paper in @BiophysReports Thanks to @JJeffet @Craggs_Lab @LabPerlson

[Fredrik Westerlund @FWesterlund]

Happy to share our comprehensive review on how nanofluidics can be used to study DNA!

https://t.co/otTU9JXywW

[Jonathan Jeffet @JJeffet]

Awesome work by Sapir @SapirM4 on the Saphyr! 🙃
Amazing how much more useful information besides the sequence is encoded on each DNA molecule...🤯

[Yuval Ebenstein @hagenom]

Ultra-long single-molecule reads with methylation and hydroxymethylation tags on the @bionanogenomics Saphyr allowed to construct the genetic and epigenetic variation maps of kidney tumor and adjacent normal kidney 1/5

https://t.co/JrBroXsuUR

[Julia Lazzari-Dean @JLazzariDean]

At the heart of every snoutscope is a sub-micron alignment between two objectives. 1°C thermal drift kicks the lightsheet out of focus and destroys your image ☹️

Our optics room swings by 5°C/day 😭...so @AndrewGYork & I made a crossbraced O2/O3 subunit to passively compensate!

[bioRxiv Biophysics @biorxiv_biophys]

MicroFPGA: an affordable FPGA platform for microscope control https://t.co/VW0J5SXEcb #biorxiv_biophys

[Ricardo Henriques @HenriquesLab]

🚨🔬😍 @afonsomendes92 @Hannah_SuperRes and @simaopc #preprint is out! An awesome collaboration with @christlet

An extensive review on structural discovery and analysis with #SuperResolution using #SingleParticleAnalysis.

Check it out in https://t.co/PgSoO4rr9I

[Jonathan Jeffet @JJeffet]

@Craggs_Lab Congrats Tim!!

[Yuval Ebenstein @hagenom]

Just finished installing a new whisky cabinet in my office. Some say it’s the only ⁦@illumina⁩ whiskey cabinet in the world…

[Cees Dekker @cees_dekker]

Today we publish a paper in @ScienceMagazine that expands nanopore readings to the proteome:
a nanopore-based scanner to read off PROTEINS at the single-molecule level! 🤩

Awesome experiments by postdoc Henry Brinkerhoff of our #CDlab, with MD simulations of @aksimentievLab

1/x

[Jonathan Jeffet @JJeffet]

@KrishnaMudumbi Thanks Krishna! If you have any questions setting CoCoS up, I'd be happy to help😊

[Jonathan Jeffet @JJeffet]

@ckinzthompson @BiophysReports @hagenom @Craggs_Lab @ArielIonescu @LabPerlson @ChizhikAlexey Thanks! Will add some pics here if you're interested. Feel free to contact if you need any help setting it up!

[Jonathan Jeffet @JJeffet]

Woohoo!!
Our 🌴CoCoS🌴 paper is going to be on the FIRST issue of @BiophysReports!
https://t.co/diMkDIfiTC

Thanks so much to @hagenom and our awesome collaborators: @Craggs_Lab @ArielIonescu @LabPerlson

The amazing illustration by @ChizhikAlexey

An explanatory thread…
1/15

[Jonathan Jeffet @JJeffet]

@Craggs_Lab Just need to land on two angles in each frame... one with RPA=180 and one with the optimal RPA for the set of markers you have

[Jonathan Jeffet @JJeffet]

@markianwallace @BiophysReports @hagenom @Craggs_Lab @ArielIonescu @LabPerlson @ChizhikAlexey Nice! Yes, I guess that will be perfect for the really high speed belt-drive motorized rotators

[Jonathan Jeffet @JJeffet]

Thanks for reading!
If you have any questions I’d be more than happy to discuss!

https://t.co/diMkDIfiTC

[Jonathan Jeffet @JJeffet]

So to summarize:
* CoCoS gives the control to set and optimise the spectral resolution.
* Keeps the FOV large and the throughput high.
* And it is simple to align and assemble so that everyone can enjoy the benefits of spectral imaging, even without being an optics expert!
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