Laura Doherty

Research Scientist @BroadInstitute and PRISM. Compound mechanism of action in cancer. Chemical Biology PhD @Harvard and @DanaFarber. UNC Chapel Hill alumna.

[The Cancer Dependency Map @CancerDepMap]

Visit us at AACR 2024!🎉

Members from DepMap and PRISM will be at booth 153. Team members will be available to answer your questions about our data, the DepMap portal and provide some tutorials of our portal tools.

We look forward to meeting you!

[Stuart Schreiber @SchreiberStuart]

This MoA will blow your mind! Amazing what small molecules are able to do. This one suggests novel future capabilities and applications.

[Caitlyn Yaede (she/her) @caitlyn_yaede]

The front page of tomorrow's @dailytarheel –

I shed many tears while typing up these heart-wrenching text messages sent and received by UNC students yesterday. Our campus was on lockdown for more than three hours.

Beyond proud of this cover and the team behind it.

[William Gibson @wgibson]

What if small molecules could allow you to alter protein localization at will?

What if they could allow you to turn on specific transcription factors?

Our new manuscript reveals bifunctional molecules to do just that. 🧵

[Alex Naka @gottapatchemall]

o no
https://t.co/xnX3TSSvvc

[Alice Ting @aliceyting]

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec.

We explore 4 intracellular and intercellular applications of TransitID:
- discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix)
- mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress
- discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery
- analysis of proteins that traffic intercellularly bw tumor cells & macrophages

Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor

https://t.co/yo4laOA14z

[Brian Liau @brian_b_liau]

Congrats to Shelby on a brilliant PhD defense unraveling genome structure at nuclear landmarks using chemical genomics! So talented, & looking forward to all her future accomplishments. Also, very fun to see the real creative side of the lab, so many genome-themed sweets!

[Milka Kostić, PhD @MilkaKostic]

Congratulations to Sara Buhrlage (@BuhrlageLab), a PI in our @DFCI_ChemBio Program on a successful launch of Entact Bio. Their goal is to develop bifunctional recruiters of deubiquitinating enzymes to modulate protein homeostasis for therapeutic benefit.
https://t.co/cqAE0mkHYV

[Laboratory of Systems Pharmacology @HarvardMedical @Harvard_Lab]

Dye Drop is a versatile, low-cost, and reproducible assay that uses sequential density displacement to obtain detailed cell cycle information in high throughput.

Want to try it? Visit https://t.co/KobpMR3zE5 to learn how!
@NatureComms https://t.co/nkRBnPWnGP

[Ryan Potts @pottslab]

A big mystery solved? Endogenous function of the CRBN Ligase revealed by Christina Woo et al @Nature!! Spoiler…it’s all about protein quality control. CRBN recognizes damaged proteins with cyclic di-peptides that mimic IMiDs! So cool👏👏 https://t.co/TzyEIdiHqZ

[Dr. T @GertrudRey]

Why do some people develop long COVID while others do not? While we still don't know for sure, some answers are beginning to emerge.

In this 🧵 I describe some groundbreaking work done by @VirusesImmunity at Yale in an attempt to answer this question. 1/

[Sahil Bloom @SahilBloom]

A framework is a way of thinking about a problem that provides clarity and improves decision making.

The most powerful frameworks I’ve found:

[Laura Doherty @LauraDohertyM]

@ubiquigent I’m glad you find it useful! The paper is also now online at eLife! https://t.co/9wyswADgCL

[Ubiquigent @ubiquigent]

In a hugely comprehensive #multiomics study by @LauraDohertyM et al., new functions of well-known DUBs and novel #drug targets have been uncovered: https://t.co/un2cHq0Ofb
Now available online, this #data is a fantastic resource for the DUB community! https://t.co/XRUu8NlMAN

[Massimo @Rainmaker1973]

Is it possible to capture the entire plane of our galaxy in a single image? Yes, but not in one exposure: Maroun Habib did in two with some planning. Top and bottom part of this image were taken six months apart and there you have it, the complete plane https://t.co/68JpIIZkew

[Joelle Straehla @jstraehlaMD]

Very excited to share our work on cancer nanomedicine now out in @ScienceMagazine. This was a really fun and collaborative project co-led by @natalie_boehnke and supported by an amazing group of scientists @kochinstitute @broadinstitute @DFBC_PedCare https://t.co/Rkgvv1wwxL

[Laura Doherty @LauraDohertyM]

@benjamingyori @DARPA @IndraSysBio @HiTSatHarvard Woohoo🥳🎉 Congratulations!

[Rita Idugboe @RitaIdugboe]

When you don’t have any new data to present at the lab meeting.

[Laura Doherty @LauraDohertyM]

So exciting to see my graduate work published! Thank you to everyone who made this paper possible! @cthoyt @benjamingyori @HiTSatHarvard @DFCI_ChemBio

Integrating multi-omics data reveals function and therapeutic potential of deubiquitinating enzymes https://t.co/9wyswADgCL