Rapoport Lab

@HarvardCellBio @HHMINEWS lab at @harvardmed. We study protein translocation across various cellular membranes and enjoy red sweaters.

[Rapoport Lab @RapoportLab]

Read our latest #research "The deubiquitinating enzyme Otu1 releases substrates from the conserved initiation complex of the Cdc48/p97 ATPase for proteasomal degradation" published in Sci Rep (2026). https://doi. org/10.1038/s41598-026-42811-6

[Rapoport Lab @RapoportLab]

Read our latest #research "A heterotrimeric protein complex assembles the metazoan V-ATPase upon dissipation of proton gradients", published with @SpringerNature in @NatureSMB
https://t.co/vvyeTAHQdj

[Rapoport Lab @RapoportLab]

Read our latest #research "Import mechanism of peroxisomal proteins with an N-terminal signal sequence", published with @SpringerNature in @NatureCellBio
https://t.co/Li6yNOwEO6

[Rapoport Lab @RapoportLab]

Excited to share our latest work led by our postdoc Dan Zhao! We uncovered how the bifunctional complex of Mnl1 mannosidase and protein disulfide isomerase (Pdi1) initiates ERAD. A long-sought ERAD reductase is finally discovered! https://t.co/0HaCgvHRVL
Huge congratulations!

[Rapoport Lab @RapoportLab]

@ninawowp @HHMINEWS https://t.co/qc9GvqH0rp

[Rapoport Lab @RapoportLab]

Mystery unveiled! Peroxisomal protein import resembles that of nuclear pore! Congratulations to Yuan and Mike for their joint effort! Thanks to @HHMINEWS for their continuous support on this project! https://t.co/gHU06JoSUB

[Rapoport Lab @RapoportLab]

We are thrilled to share our new work published in Nature today reporting a long-sought peroxisomal retrotranslocation channel formed by a ubiquitin ligase complex. Phenomenal work by our postdocs Peiqiang Feng, Xudong Wu and collaborators.
https://t.co/e9XeqCfdQC

[Rapoport Lab @RapoportLab]

@ZhejianJ @jrengen @GygiLab

[Rapoport Lab @RapoportLab]

Our story on substrate translocation mechanism by Cdc48 is online now. Check out this Christmas present here https://t.co/ubjo0JTd1y. Big thanks to everyone for their excellent contributions.

[Rapoport Lab @RapoportLab]

@Shenhav_Lab @ZhejianJ @jrengen @GygiLab We don't think the Cdc48 complex has any substrate specificity. According to our IP mass spec experiment performed in yeast lysate, most proteins with a K48-linked ubiquitin chain can bind the ATPase complex.

[Rapoport Lab @RapoportLab]

Check out our new preprint on the translocation mechanism of the holy-grail Cdc48 ATPase. Congrats to @ZhejianJ and Hao Li, as well as to our wonderful collaborators the Engen lab @jrengen , the Marto lab, and @GygiLab !

[bioRxiv @biorxivpreprint]

Translocation of polyubiquitinated protein substrates by the hexameric Cdc48 ATPase https://t.co/6c7gopjfRp #bioRxiv

[Rapoport Lab @RapoportLab]

Legobodies are online now, with full sequences available! Have fun!👉https://t.co/WmdHaXRvbV

[Rapoport Lab @RapoportLab]

@JiaweiZhao5 The sample with only the nanobody won't even survive till the grid preparation step-> heavy aggregates

[Rapoport Lab @RapoportLab]

Check out our new preprint on Making the nanobody Big again for Cryo-EM, spearheaded by Xudong Wu (will start his own lab at Westlake University soon) in the lab.👉
https://t.co/nmpY3ynAUh

[Jeeyun Chung @JeeyunC]

Excited to share our study on the mechanisms underlying selective autophagy of lipid droplets. We showed that Troyer syndrome protein spartin is a key to this! A wonderful collaboration with @synapsepark and @TalleyJLambert. Amazing support:@DamonRunyon https://t.co/e0fSLox5oo

[Rapoport Lab @RapoportLab]

Check out our new review on protein translocation through a distorted lipid bilayer.

https://t.co/MfzW7D0cpC

[Rapoport Lab @RapoportLab]

@A_Amunts It is tricky, but we are working on solving the structure by cryo-EM.

[Rapoport Lab @RapoportLab]

Want to know what will happen if a membrane protein is too good at generating curvature? Check our new paper
https://t.co/J3TSislWFW

[Rapoport Lab @RapoportLab]

@tweet
Want to know what will happen if a membrane protein is too good at generating curvature? Check our new paper https://t.co/J3TSislWFW

[Beckmann Lab @BeckmannLab]

#CryoEM structure of the post‐translational Sec #translocon with substrate bound and X-ray/HDX of the Sec62 subunit.
➡️ @embojournal paper online now!
Congrats to @tw_weng, @jingdongcheng, @BangeBalcony and everyone involved! 🎉👏
https://t.co/9pvJ6IEJuI