Roman Doll

DPhil Student in Medical Sciences @UniofOxford @MRC_WIMM @jdavieslab

If it has a genome, we can do genome editing on it, right? 🧬

[Roman Doll @RomanMDoll]

Our work from my master’s project exploring cytosine base editors for gene editing in Drosophila is now published @ScienceAdvances (my first first-authorship🎉)

https://t.co/hdiDCcYbyP

There are several additions since the preprint, so read along for a 🧵with the main points:

[Ben Kleinstiver @BKleinstiver]

Check out our @biorxivpreprint describing our efforts to treat a severe vascular disease 🫀:
1️⃣ Optimization of a target-specific base editor🧬
2⃣ A *must avoid* bystander edit prevalent with WT SpCas9 BEs! 🙅‍♂️😱
3⃣ Substantial extension of lifespan following in vivo base editing! 🐁✅

A 🧵 below with more details!
@CGM_MGH @MGH_RI @MassGeneralNews @harvardmed
https://t.co/OZlW9ntsQw

[Joana Ferreira da Silva @FerreiraSilvaJ]

Thrilled to see our click editing paper out today in @NatureBiotech! 🚀 Click Editors (CEs) are a new class of genome writers which combine DNA polymerases and HUH endonucleases with RNA-programmable nickases for precise edits without DNA DSBs🧬✨ https://t.co/CXqicVhjZU

[Connor Tou @connorjtou]

Today in @NatureBiotech, we describe first-gen click editors (CEs), which couple the advantageous properties of DNA-dependent DNA polymerases and HUH endonucleases with RNA-programmable nickases for precise and versatile human genome editing. 🧬

https://t.co/XwQs0vIhGv

[Ben Kleinstiver @BKleinstiver]

SpRYgests now enabled via purified SpRY protein through @NEBiolabs, available here:
https://t.co/X1vKDlzvqe

Super cool to have this option 'live' now - congrats to @russelltwalton @katieachristie @RomanMDoll & many others who contributed.

Happy SpRYgesting 😃

[Siamak @Sia_hai]

Pleased to share our latest preprint identifying a GTPase cascade that modulates regional Wnt signalling in the intestine:

https://t.co/oPmfz3XJtF

[Dina D. Pomeranz 🟣 @DinaPomeranz]

We tend to massively idealize our memories or imaginations of the past. The good old times never existed, except in our minds.

[Charlotte Brierley @CKBrierley]

Heading home from #ASH23? 👩‍💼🌞🌮Stuck in a line at security or facing a long layover? ✈️In withdrawal from all the amazing science? 🧪🧬 Read our preprint from my PhD work @UniofOxford @MRC_WIMM presented in the MPN session yesterday! https://t.co/uH3dzJ5ueD 1/n

[Grace Meaker @grace_meaker]

Have you ever wondered which methods are out there to culture haematopoietic stem cells? Here we review progress and challenges facing the field today! https://t.co/VXffqpBjqc
@awilkinsongroup @MRC_WIMM #exphem #hsc

[Ben Kleinstiver @BKleinstiver]

Delighted to share that our work to develop a base editor-mediated genetic treatment for SMA is now published in @natBME 💉💉🧬🎉
Congrats 👏 to @Christiano_RRA who led this work & all co-authors
@CGM_MGH @MGH_RI @MGHNeurology @MGHPathology @harvardmed
🧵
https://t.co/CsK85TzLZ8

[Nicolas Mathis @nicopmat]

🚨New preprint🚨Today, we're excited to share our latest study, "Predicting prime editing efficiency across diverse edit types and chromatin contexts with machine learning" via @biorxivpreprint! @schwanklab @krauthammerlab @bvansteensellab @UZH_Science
https://t.co/CfpKr6hthm
🧵

[Ishmail Abdus-Saboor @IshmailSaboor]

Today's Nobel is a reminder to focus on the work and not the journal. The key publications cited by the committee were published in Immunity, Molecular Therapy, and Nucleic Acids Research. Respectable journals, but not the Big 3. The discoveries are what's important, not a name.

[Stefanie Stantcheva @S_Stantcheva]

Zero-sum thinking is a key mindset that shapes how we view the world. Excited to share a new paper on the roots and consequences of Zero-sum thinking with @sahilchinoy, @DrNathanNunn @SMGSequeira. A summary thread🧵1/23 https://t.co/wqk3BQ5lGZ

[Roman Doll @RomanMDoll]

Threads like this one are what makes this platform worthwhile for me! Super interesting read on zero-sum thinking.

[Stefanie Stantcheva @S_Stantcheva]

Zero-sum thinking is a key mindset that shapes how we view the world. Excited to share a new paper on the roots and consequences of Zero-sum thinking with @sahilchinoy, @DrNathanNunn @SMGSequeira. A summary thread🧵1/23 https://t.co/wqk3BQ5lGZ

[Ben Kleinstiver @BKleinstiver]

We’re excited to share our work developing a new gene editing approach that we’re calling #clickediting 🖱️✴️🧬.

Click editors (CEs) utilize DNA-dependent polymerases to write bespoke edits into the genome. https://t.co/E6DOZD1CWp
@CGM_MGH @MassGeneralNews @MGH_RI @harvardmed

[Joana Ferreira da Silva @FerreiraSilvaJ]

Today in @bioRxiv, we describe Click Editors (CEs) which combine DNA-dependent DNA polymerases, HUH endonucleases and RNA-programmable nickases for versatile genome editing without double-strand breaks 🧬. A study co-led with @connorjtou. A 🧵 (1/14):
https://t.co/lSO6Kk0Rk9

[Connor Tou @connorjtou]

Today in @biorxivpreprint, we introduce Click Editing - which combines DNA-dependent polymerases, HUH endonucleases, and RNA-programmable nickases for versatile human genome editing without double-strand breaks. Co-led with @FerreiraSilvaJ 🤝🧬 (1/15)

https://t.co/MXhwBQjR9m

[Li-Hsin Chang @Lihsin0507]

Very happy and honored to present our work from my postdoc project in @DaanNoordermeer lab @I2BCParisSaclay on CTCF clustering at TAD boundaries and the novel multi-contact 3C assay: Nano-C – now published @NatureComms.

https://t.co/6l1d6ID0PL.

A nice 🧵 written by Daan 👇

[Martina Fiumara @fiumara_martina]

I am truly excited to share our latest work about the upsides and pitfalls of base and prime editing in #Hematopoietic #stemcells.

Thanks to @ferrari_sam93, the @NaldiniLab and all the collaborators for the huge effort.
@Telethonitalia @SanRaffaeleMI
https://t.co/XdwW4VApCT

[Alba Meira @AlbaRMeira]

🔥And it’s out!🔥 Today, we report in @NatureGenet how chronic inflammation drives the selective expansion of TP53-mutant cells in the blood, leading to the development of a deadly type of leukemia.

https://t.co/PliXCjEJ5N

[Roman Doll @RomanMDoll]

@mn_whittaker @katieachristie @ScienceAdvances Yeah, another one😁 Thanks Madelynn!

[Roman Doll @RomanMDoll]

Our work from my master’s project exploring cytosine base editors for gene editing in Drosophila is now published @ScienceAdvances (my first first-authorship🎉)

https://t.co/hdiDCcYbyP

There are several additions since the preprint, so read along for a 🧵with the main points: