Trevor S. Wendt

Cerebrovascular Physiology and Pharmacology Post-Doc

[Trevor S. Wendt @TrevorSWendt]

@RJGonzalesPhD @lunduniversity So happy to be able to share with the world!

[Circulation Research @CircRes]

Arkelius et al found rats treated with #rt-PA after stroke, additional #LOX-1 and #MMP-9 inhibition reduced number & severity of #hemorrhages, & better maintained neurological function compared with rt-PA alone. Learn more at https://t.co/nqI19bXGjr

@TrevorSWendt

[Taben M. Hale, PhD @TMHalePhD]

Thrilled to share this exciting work by @RJGonzalesPhD and @TrevorSWendt! @uazmedphx @uazmedtcrc @UAZBIO5

[AJP-Cell Physiology @AJPCellPhys]

Final #ArticleinPress this week comes from @uazmedphx. Ozanimod Differentially Preserves Human #Cerebrovascular #Endothelial Barrier Proteins and Attenuates MMP-9 Activity Following In Vitro Acute Ischemic #Injury (Trevor S. Wendt and Rayna J. Gonzales):

https://t.co/VlNsKIZSwz

[biorxiv_physio @biorxiv_physio]

OxLDL/LOX-1 Mediated Sex, Age, and Cell Dependent Alterations in Mouse Thoracic Aortic Vascular Reactivity https://t.co/mS2u00kHbd #biorxiv_physio

[Nature Portfolio @NaturePortfolio]

A Review in @NatRevNeurol provides detailed insight into how the gut microbiota influences the immune system and discusses the accumulating evidence that the gut microbiota is an important factor in multiple sclerosis pathogenesis. https://t.co/Y8ENGzs9xU

[Mizushima Lab (UTokyo) @MizLabUT]

Monitor autophagic flux in mammalian cells easily with our new method, now out in @eLife! Pulse-labeled HaloTag reporters are processed in lysosomes, releasing proteolysis-resistant labeled Halo. Free labeled Halo amount = autophagic flux! →western/in-gel fluo/microsc./flow cyto

[Hao Yin @HaoYin20]

#CapillaryStalling caused by #EndothelialCell #Glycocalyx attrition

#OpticalCoherenceTomography+2PM

Heparinase+Hyaluronidase, or🐭#VascularDementia induced by Zoletil+xylazine i.p

Mainly Leukocyte plugging

Dr Wang-Yuhl Oh & Yong Jeong labs @_JCBFM 2022
https://t.co/4wsfZZpd4E

[Hao Yin @HaoYin20]

#EndothelialCell #Mechanobiology

#Mitophagy machinery (p62/PINK1/SMDT1) is required for #LaminarShearStress-activated MEKK2/3-MEK5-ERK5-KLF2 axis

p62 #Autophagy adaptor physically scaffolds with MEK5

Dr. Brian Coon & Martin Schwartz labs @JCellBiol 2022
https://t.co/ezfs24CFqG

[Hao Yin @HaoYin20]

Cells of the #BloodBrainBarrier: An Overview of the Neurovascular Unit in Health & Disease

Relearn BBB+#NeurovascularCoupling from this very enjoyable review by
Dr. Heather McConnell @neurovessels
Dr. Anusha Mishra @Mishra_CBF_Lab
Methods Mol Biol 2022
https://t.co/T3Uw7BSgVB

[Hao Yin @HaoYin20]

An exquisite, quantitative map of🐟zonated #EndothelialCell morphodynamics🤠

Directional migration+Mitosis 26-38hpf

#WallShearStress
aISV vs vISV
in situ vs in silico

Dr. André Rosa & Holger Gerhardt lab @Dev_journal 2022 @andreangeloo @holger_gerhardt
https://t.co/5HdigcmQBK

[Hao Yin @HaoYin20]

Details matter

A super-detailed protocol to stain & quantify 3D (150-μm slice)🐭brain vasculature
Density
Branching
Tortuosity

Learning a new trick, I will try fish gelatin in IHC next😆

Dr. Baptiste Lacoste lab Neurophotonics 2022 @BaptisteLacost3
https://t.co/RRVWwHmMqH

[Hao Yin @HaoYin20]

Translating the Force - mechano-sensing GPCRs

A rich collection👇
AT1R
PAR1
S1PR1
M3R
FPR
PTH1R
B2R
GPR68
H1R
APJ
+Adhesion GPCRs

🤠Crosstalks with mechanosensitive ion channels

Dr. Ines Liebscher lab @AJPCellPhys 2022 @Caroline_Wilde2 @LiebscherLab
https://t.co/iMtfRLr6eP

[Hao Yin @HaoYin20]

🐭ACE2 expression spectrum

Abundant ACE2 in #Pericyte (CNS/Heart/Pancreas...),
SMC subsets but Not #EndothelialCell

In Lung, ACE2 mainly detected in AT2 & multiciliated airway epithelium

Dr Urban Lendahl & Christer Betsholtz labs @stemcellreports 2022
https://t.co/F3Zl726njm

[Hao Yin @HaoYin20]

#EndothelialCell Notch KO <3 mo -> Artery #TertiaryLymphoidStructure🐭Kidney/Lung/liver

Enthralling🤩Artery acquiring #HighEndothelialCell phenotype!

Arterial mural cell phenotype?
Origin of TLS FRC?😋

Dr. Florian Limbourg lab @NatureComms 2022 @fleig_s
https://t.co/RvOYzyaYzq

[Hao Yin @HaoYin20]

Trafficking in blood vessel development

A nice review on #RabGTPase #VesicleTrafficking machinery in #EndothelialCell #Angiogenesis

Sprouting
Junctional dynamics
Lumenogenesis

VEGFR2/VE-cad/Notch/Integrin/WPB

Dr. Erich Kushner lab Angiogenesis 2022
https://t.co/y9ZSXrZqYd

[Hao Yin @HaoYin20]

Endothelium protects #SmoothMuscleCell from H2O2-induced, caspase-/Ca2+-dependent Death in pressurized🐭#PosteriorCerebralArtery

EC-derived cytoprotective factor for SMC?
NOT #GapJunction #CarbonMonoxide #NitricOxide

Dr Steven Segal lab Acta Physiol 2022
https://t.co/ojVb2CzCbi

[Raleigh M. Linville @LinvilleRaleigh]

Microvascular stabilization via blood-brain barrier regulation prevents seizure activity | @NatureComms
So much to unpack here 🧐 Check it out for all things #claudin5! #bloodbrainbarrier #Epilepsy
https://t.co/cNu5yQGo0G

[Nature Cardiovascular Research @NatureCVR]

Research| @Paul_P_Cheng et al. show that #SmoothMuscleCell-specific deletion of #Smad3 resulted in increased SMC-derived #chondromyocytes, increased outward remodeling and #plaque #calcification. @StanfordMed| https://t.co/NDSu9Cs6cj

👉https://t.co/F7sQ64hQPu

[Hao Yin @HaoYin20]

Propagation of Lipotoxicity-induced #SkeletalMuscle #ERStress via #ExtracellularVesicle packing long-chain #Ceramide

#MyoTwitter

Dr. Lee Roberts lab @NatureComms 2022 @Roberts_Lab
https://t.co/bdl21KqFp5