Yi (Eason) SHEN 沈溢

Scientist & Protein Engineer | Husband & Dad

[Abhi Aggarwal, MD @abhi_aggarwal1]

Thrilled to share #TGECO1, a new blue-shifted Ca2+ indicator for imaging neural activity, freshly published in Neurophotonics! Large 2PX, great for multiplexing!

https://t.co/eBgbKNjlak

@Campbell_FPs @easonyshen @PodgorskiLab @AllenInstitute @HHMIJanelia @UCalgary

[Ahmed Abdelfattah @Abdelfattah_Lab]

We are recruiting for an open Postdoc position. Interested in developing molecular tools? Our research is at the intersection of neuroscience, biochemistry, and biophysics. Please spread the word. Reach out via email if interested!
@BrownUNeuro @CarneyInstitute

[Joachim Goedhart @joachimgoedhart]

Red fluorescent proteins engineered from green fluorescent proteins
https://t.co/GFdMPKssd0

[Abhi Aggarwal, MD @abhi_aggarwal1]

Thrilled to share a new teal Ca2+ indicator! #TGECO is a blue-shifted biosensor with high SNR & enhanced 2P absorption, great for #multiplexing in vivo!🧵

See preprint below⬇️
https://t.co/wjbnlseqj6

@Campbell_FPs @easonyshen @PodgorskiLab @AllenInstitute @HHMIJanelia @UCalgary

[Joachim Goedhart @joachimgoedhart]

Photoswitchable binders enable temporal dissection of endogenous protein function
https://t.co/Gm8vUvdepL

[Helen Farrants @HelenFarrants]

Our #chemigenetic #fluorescent calcium indicator WHaloCaMP is now out on #bioRxiv : https://t.co/vPGSCY55wU WHaloCaMP can be used to report on neuronal activity in flies, zebrafish and mice. WHaloCaMP is great for #multiplexedimaging. A 🧵:

[Yi (Eason) SHEN 沈溢 @easonyshen]

@HSchihada Thank you for your interest in using LSSmApple! We distribute the plasmid via @addgene. Pls order from there!

[Yi (Eason) SHEN 沈溢 @easonyshen]

Introducing LSSmApple, a long Stokes shift red fluorescent protein, enabling the construction of 2nd gen dual-FP Matryoshka sensors for high throughput quantitative analysis with minimal spectral bleed-thru. https://t.co/EAwexptnlz

[Yi (Eason) SHEN 沈溢 @easonyshen]

It's been a great pleasure to work with @jejike1 @MayuriSadoine Tom Kleist @healthy_crop & others on this project! Stay tuned for more follow-ups. LSSmApple plasmid is available on @Addgene https://t.co/9PK0oR9HHN

[Andrew York @AndrewGYork]

We discovered a surprisingly easy way to control the brightness of fluorescent proteins via magnetic fields:

https://t.co/zxPPsd14zP

This raises fascinating scientific questions about the mechanism, and tantalizing technological opportunities for bioengineering.

[Prof. Michael Lin @MichaelLinLab]

Today in @naturemethods: our new positively tuned voltage indicator, ASAP4e, for extended electrical recordings in the brain.

ASAP4e achieves greater responsivity (>200% per 100mV vs 50% for other fluorescent voltage sensors) and better photostability.

https://t.co/wOq1fAtx5h

[Abhi Aggarwal, MD @abhi_aggarwal1]

iGluSnFR3 is published! Check it out⬇️
https://t.co/hQC58oNxj2

We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission.

This is #iGluSnFR3 in action detecting minis w/ TTX.
@PodgorskiLab @HHMIJanelia @AllenInstitute

[Yusuke Nasu @NasuLab_IBC]

The latest versions of genetically encoded L-lactate biosensors, LACCO series, are out in bioRxiv: a second-generation green fluorescent extracellular biosensor eLACCO2.1 and a red fluorescent intracellular biosensor R-iLACCO1. #lactate #biosensor https://t.co/ORvKO2bn3u

[Yi (Eason) SHEN 沈溢 @easonyshen]

@Shuce_Z @ualbertachem @UAlberta @Campbell_FPs @Amgen Congratulations Shuce！

[Ben Campbell @lysergicdesigns]

Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵https://t.co/hyUvgeno85

[PLOS Biology @PLOSBiology]

#Potassium ions are an essential electrolyte in all biological systems. @easonyshen @Campbell_FPs &co describe high-performance genetically encoded K+ sensors to enable in vivo measurement of [K+] across multiple model organisms #PLOSBiology https://t.co/1ahS7cgubH

[Yi (Eason) SHEN 沈溢 @easonyshen]

@P_M_Mueller Our new mScarlet-I & mWatermelon🍉 pair might be of interest to you? We also tuned the affinity a bit to optimize the FRET efficiency. https://t.co/VYpBYPap32

[Yusuke Nasu @NasuLab_IBC]

Are you interested in a red fluorescent genetically encoded biosensor for extracellular L-lactate? Please check out our preprint on R-eLACCO2! https://t.co/865RtNc1id
Thank you so much, everyone contributing to this project! @Campbell_FPs

[Alexey Chizhik @ChizhikAlexey]

Inspired by a fantastic sculpture recently made by @mtyka. #SciArt

[Yi (Eason) SHEN 沈溢 @easonyshen]

Clearing the lab fridges today and found these gems! Happy #fluorescencefriday