Gardner Lab

News from Dr. James Gardner's lab @UCSF, @UCSFtransplant, &
@Atucsf, focusing on advances in basic immunology and immune tolerance.

[Ansu Satpathy @Satpathology]

Excited to introduce CRISPR-All! – a flexible, high-throughput framework that combines virtually any perturbation modality (CRISPRko, CRISPRi, CRISPRa, shRNA, knock-in, base/prime editing, etc.) in the same cell, at scale.

Paired with single-cell RNA-seq @10xGenomics and rich phenotypic readouts, it unlocks massively combinatorial genetic screens and new cell states previously out of reach.

Outstanding work led by @Roth_Lab @AustinMHartman

https://t.co/hU3OKDfMSd

[Gardner Lab @gardnerlabUCSF]

@DoctorQMd @UCSFSurgery @UCSFTransplant @UCSF @UofCalifornia @UCSFMedicine @SandyFeng8 @PheoSurgeon @TraumaDocSF @UCSFHospitals Thanks so much @DoctorQMd, appreciate the support from such an inspirational surgeon-scientist!

[Gardner Lab @gardnerlabUCSF]

@UCSFSurgery @UCSFTransplant @UCSF @UofCalifornia @UCSFMedicine @SandyFeng8 @PheoSurgeon @TraumaDocSF @UCSFHospitals Thanks so much to @UCSFSurgery, @UCSFTransplant, @AtUcsf, and of course the @theNCI for the amazing support. This work is a close collaboration with the @SpitzerLab, who have been amazing partners, led by @sguldberg97, @mattarvedsonphd, and transplant surgical fellow Alex Gupta.

[Suneil Koliwad @SuneilKoliwad]

Congrats to my former @UCSF next door neighbor Sonja Schrepfer and colleagues on taking a key step towards restoring beta cell function in people with T1D without immunosuppression. This moves us towards a cure! @Sana_Biotech @NEJM @AtUcsf

[UCSF Surgery @UCSFSurgery]

🌟Exciting ‼️@UCSF Transplant Surgeon Dr. Shareef Syed has been selected as an Associate Member of the @AmCollSurgeons Academy of Master Surgeon Educators. This Fall, he'll be officially inducted into the academy in Chicago. Congratulations, Dr. Syed🏅

[UCSF Surgery @UCSFSurgery]

Achieving and maintaining harmony between the liver and the host immune system is critical for liver graft health. Dr. @SandyFeng8, Vice-Chair of Research has earned a $7.2M @NIAIDNews grant to decode the tipping point between calm and crisis in children with liver transplants‼️

[Kyle Cromer @M_Kyle_Cromer]

Just released our pre-print on bioRxiv for UNCOVERseq, an improved off-target discovery platform for Cas9 and base editors! Great collaboration with the awesome team at IDT @idtdna
https://t.co/o39DHgvuUi

[UCSF Surgery @UCSFSurgery]

‼️We’re happy to share that @UCSF Fellow Dr. Hillary Braun is staying in San Francisco and will be joining the Division of Transplant Surgery @UCSFTransplant as Assistant Professor of Clinical Surgery after completing her Abdominal #Transplant Surgery Fellowship👏👏

[Ryan Corces @ryancorces]

CHOIR is officially published @NatureGenet. No more arbitrary decisions in clustering. Add this to your single-cell toolbox - it will save you so much heart ache. And now it can scale to millions of cells. Compliments of the very talented @cathrine_sant.

[Chris Hsiung 熊 @hsiung_chris]

check out this amazing free software for flow analysis. say goodbye to flowjo.

[Suneil Koliwad @SuneilKoliwad]

From “preprint” to “in press”!

Congrats @MValdearcos @RachelLippert and the rest of our outstanding team. @AtUcsf @UCSF #microglia #hypothalamus

Microglia mediate the early-life programming of adult glucose control: Cell Reports https://t.co/ORt1WPDMZZ

[Mohamed Adam, MD, FACS @mohamedadamMD]

Proud of our fantastic @UCSFMedicine medical student Han Yin @hansyin96 who presented our combined supra-&infra-mesenteric robotic technique for mid D3 tumors #SAGES2025. A complex topic, masterfully delivered.👏🎉
@HPB_Surgeon @UCSFSurgery @UCSFGSResidency

[American Journal of Transplantation @amjtransplant]

Spend some time reading the March Literature Watch "A trip down (obesogenic) memory lane" by Sarah Alshammery and Natasha M. Rogers: https://t.co/GHm1spaq48

[Justin Eyquem @j_eyquem]

🚨Excited to share our latest work, now out in @NatureBiotech ! We introduce SEED-Selection, a powerful method that enables high-efficiency enrichment of T cells edited at multiple loci in a single step 🧵👇
🔗 https://t.co/5Dh4hQIfDn

[UCSF Surgery @UCSFSurgery]

‼️Dr. Holger Willenbring, @UCSFLiverCenter Director and @UCSFTransplant Surgeon Dr. Garrett Roll explore AAV capsid prioritization in normal and steatotic human livers maintained by machine perfusion in their latest paper published in @NatureBiotech 👉 https://t.co/MbON3hHRT2

[UCSF Immunologists for Gender Equity @IgEquity]

Our final section, Cell Engineering🧫🧬 @HuiCharlotte of @j_eyquem/@yimmieg labs will start things off, followed by Lauren Tabor of the Bruno lab and Marta Borowska of the Garcia lab! We are stoked for our keynote Dr. Jennifer Doudna @doudna_lab 🤩 can’t wait to see you there!

[Gardner Lab @gardnerlabUCSF]

RT @BrisaPalikuqi: Please RT:

📢 Exciting Opportunity! 📢

The Brisa Palikuqi Lab at Yale Med is hiring a Postgrad Research Associate in Ste…

[Ansu Satpathy @Satpathology]

Happy to share our new work describing DESynR genes!

The evolution of new human protein-coding genes relies on repurposing protein domains from ancestral genes, rather than de novo sequence creation. New genes evolved from old parts, and while we have methods that enable high-throughput generation and screening of base-pair level sequence evolution (mutagenesis, directed evolution, CRISPR, etc), we lack similar scalable technologies for domain-level evolution.

We developed a high-throughput combinatorial molecular assembly method to generate thousands of barcoded novel genes from the domain building blocks of natural genes, termed DESynR (Domain Engineered via Synthesis and Recombination) genes.

[Anna Molofsky @AnnaMolofskyLab]

Our latest paper with @Molofsky_lab is out! tweetorial to follow. Group 2 innate lymphoid cells promote inhibitory synapse development and social behavior | Science https://t.co/u2fQ2j2OeN

@jerika_barron @ Nick Mroz

[Rockefeller U. Press @RockUPress]

In @JExpMed Tam, Cyster et al @UCSF report that a lymphoma-associated gain-of-function GPR34 variant promotes immune cell accumulation in the peritoneal cavity & demonstrate that this accumulation requires the lysophosphatidylserine-generating enzyme PLA1A https://t.co/Avd8zPO2YI