Top Tweets for #mTurquoise2
Fantastic to see #mTurquoise2 expressed in monkey brains together with #mNeonGreen and #mRuby2
https://t.co/Scp4XVkeOJ
#mTurquoise2 has a 'relatively high QY' - the highest quantum yield among monomeric fluorescent proteins, to be precise.
A couple of words on #mTurquoise2 in a review of Quantum Biology: An Update and Perspective
https://t.co/eMGErIPiH5
There are a few 'blue' colonies that represent the original #mTurquoise2 template. We think that bright colonies reflect T203Y and dimmer colonies are T203H - sequencing will tell.....
Our first attempt at In Vivo Assembly (IVA) for site-directed mutagenesis: residue T203 was targeted in #mTurquoise2 and changed to Y or H - efficiency >90%
Primer design according to: https://t.co/0MKNVaVkuW
Huh? Same file openend in ImageJ (left) versus @FijiSc (right), showing #mTurquoise2 labeling of the endoplasmic reticulum
It turned out that tdTomato is a good FRET acceptor and we generated an EPAC sensor that uses #mTurquoise2 and tdTomato as a FRET pair. The Kd for cAMP is 4µM and a non-binding mutant (R279L) hardly responds
The original EPAC-based cAMP sensor uses a #mTurquoise2-Venus FRET pair. However, Venus is pH sensitive, which is problematic in yeast. Therefore, we were looking into acceptor with a low pKa
@mpbruchez @samuelconnell @TalleyJLambert @erin_rod_phd @MillerChemBio @NathanShaner @HenriquesLab The spectrally identical #mTurquoise2 has a slightly higher brightness in cells and a much better photostability.
The improved photostability is independently observed in other labs, but may depend on the power and illumination method.
Note the superior photostability of #mTurquoise2 relative to mCerulean3
This has been documented as well in our original study:
https://t.co/BIjKvP21bp
@timisstuck @NathanShaner If you want a tried and tested acid-resistant FP try mCherry, tagRFP(-T) or #mTurquoise2
Image of mixed fluorescent beads loaded with either #mTurquoise2 or LSS-SGFP2
(Excitation with light of 405nm, picture taken through the eye-piece of the microscope)
Generating fluorescent beads by loading Ni-NTA sepharose with purified Fluorescent Proteins
#mTurquoise2, GFP, YFP
Generating fluorescent beads by loading Ni-NTA sepharose with purified Fluorescent Proteins
#mTurquoise2, GFP, YFP
@andrewng_synbio mEGFP would be my first choice: Truly monomeric and spectrally compatible with virtually any microscope.
Next to that I can recommend #mTurquoise2, mNeonGreen, mScarlet-I, mCherry (but check filters and excitation source first)
@wormsense @Vermeer_Lab @pedrosaurio #mTurquoise2, we've gotten pretty good results:
https://t.co/K2iBKfqqsW
Positive control available from @addgene: https://t.co/foaMirhDvt
Last Seen Hashtags on Sotwe
snapchat
Seen from United Kingdom
maledom facesitting
Seen from United States
ryankeely
Seen from Australia
minichat
Seen from United States
desi ai
Seen from India
Teen vidéo
Seen from Netherlands
Tiktok
Seen from Indonesia
หนองกุงศรี
Seen from Indonesia
cumcontrol
Seen from Japan
dicktok
Seen from Malaysia
Most Popular Users
Elon Musk 
@elonmusk
241.5M followers
Barack Obama
@barackobama
118.9M followers
Cristiano Ronaldo
@cristiano
114.6M followers
Donald J. Trump
@realdonaldtrump
111.8M followers
Narendra Modi
@narendramodi
107.1M followers
Rihanna
@rihanna
98.7M followers
NASA
@nasa
92.3M followers
Justin Bieber
@justinbieber
91.8M followers
KATY PERRY
@katyperry
90.1M followers
Taylor Swift
@taylorswift13
84M followers
Lady Gaga
@ladygaga
75.5M followers
Virat Kohli
@imvkohli
73.5M followers
Kim Kardashian
@kimkardashian
70.9M followers
YouTube
@youtube
68.8M followers
Neymar Jr
@neymarjr
66.5M followers
Bill Gates
@billgates
65.3M followers
Selena Gomez
@selenagomez
63.1M followers
The Ellen Show
@theellenshow
62.2M followers
CNN
@cnn
61.7M followers
X
@x
60.7M followers