Bukau Lab

Our research group @ZmbhH is interested in protein biogenesis and quality control. This account is run by the members of the @BukauLab.

[Bukau Lab @BukauLab]

Our first 2021 paper reveals the prevalence and mechanism of a co-translational protein assembly mechanism in human cells: co-co assembly.
Congratulations to the co-co team @KaiFenzl, @matildebertolin and @guenter_kramer!
https://t.co/dTUfKZ06If
@Sciart by @munafomarzia
1/6

[Andrii Melnyk @MelnykAndrij]

What shall Germany deliver next? A good question posed by @faznet, thanks 🙏🏻 Well, the answer is obvious:
Ukraine desperately needs 🇩🇪 fighter jets Tornados & Eurofighters💪
Dear German Government, please do not hesitate
Just do the right thing!
https://t.co/KL7o31erfX

[Bukau Lab @BukauLab]

Our team will combine advanced ribosome profiling, cryo-EM and single-molecule experiments, to study how multiple ribosomes, mRNAs, nascent chains, chaperones and other factors form dynamic super-assemblies that act as coordinated protein complex assembly lines.

[Bukau Lab @BukauLab]

Great news! Together with @sjtans & @The_Ban_Lab, we are among this year's recipients of a Synergy Grant from @ERC_Research. This marks the beginning of an exciting journey into a mechanistic analysis of the biogenesis of protein complexes 🧬

[Bukau Lab @BukauLab]

Hier eine schöne Allegorie wie das von @LabJoazeiro und @PfefferCryoLab beschriebene MutS2 Protein fehlerhafte Proteinbiosynthese anhand kollidierter Ribosome erkennt und verschiedene Qualitätskontrollmechanismen initiiert bevor das fehlerhafte Protein der Zelle schaden kann.

[Uni Heidelberg @UniHeidelberg]

Auffahrunfall auf der „Ribosomen-Autobahn“: Wissenschaftler identifizieren bakterielles Protein, das „stehengebliebene“ Ribosomen erkennt und rettet
@ZmbhH

https://t.co/sEuA2KHraY

[Stefan Pfeffer @PfefferCryoLab]

How do cells fire up the #RQC engine? Check out our structural and mechanistic blueprint for bacterial #ribosome collision sensing by MutS2, out today in @Nature. MutS2 senses collisions and splits ribosomes to initiate RQC!

https://t.co/SNl3eK2Qkw

[Bukau Lab @BukauLab]

B. subtilis MutS2/RqcU deals with stalled ribosomes by binding collided disomes, recruits the RNA degradosome and initiates #RQC by #ribosome splitting. Happy we could contribute to this work led by @LabJoazeiro in collaboration with @PfefferCryoLab https://t.co/US3DJqGyc7

[ZMBH - Center for Molecular Biology Heidelberg @ZmbhH]

A team of researchers led by ZMBH's PIs C. Joazeiro @LabJoazeiro and S. Pfeffer @PfefferCryoLab discovered how the bacterial MutS2 protein prevents damage by stalled ribosomes! Further details in Nature https://t.co/ZMiSZDxPVb

[Bukau Lab @BukauLab]

@DellaDavid_Lab @BabrahamSignal1 @BabrahamInst This is wonderful, Della. Have a great start in your new lab

[ZMBH - Center for Molecular Biology Heidelberg @ZmbhH]

A new study by the @bukaulab in @CellReports sheds light on role of Nt acetylation in eukaryotes https://t.co/eMd9VeQEkv

[ZMBH - Center for Molecular Biology Heidelberg @ZmbhH]

Comparative SUMO proteome analysis identifies novel SUMO-controlled regulators in EGFR signaling - paper by a team led by @frauke_melchior https://t.co/IOVPBkFakV

[Bukau Lab @BukauLab]

@bioSavinov Hi Andrew, that's a good question! Indeed, our method may enrich for more stable interactions which survive the purification procedure. Some additional work in the lab suggests we are not missing many disomes by DiSP, but it is definitely worth further investigation :)

[Bukau Lab @BukauLab]

@AChemSynBio Thanks Abhishek, glad you enjoyed our work! That's a good question, some heteromeric subunits are indeed hits of our screening, but they await deeper investigation!!

[Bukau Lab @BukauLab]

More details @ScienceMagazine: https://t.co/dTUfKZ06If
6/6

[Bukau Lab @BukauLab]

Our first 2021 paper reveals the prevalence and mechanism of a co-translational protein assembly mechanism in human cells: co-co assembly.
Congratulations to the co-co team @KaiFenzl, @matildebertolin and @guenter_kramer!
https://t.co/dTUfKZ06If
@Sciart by @munafomarzia
1/6

[Bukau Lab @BukauLab]

Our data suggest that co-co assembly generally involves neighbouring ribosomes on one mRNA, thereby ensuring specific homomer formation of isoforms and structurally-related proteins which are highly prone to promiscuous interactions inside cells.
5/6

[Bukau Lab @BukauLab]

@Marius_Lemberg @matildebertolin @KaiFenzl @ZmbhH Thanks Marius!! Happy new year 🥳

[Bukau Lab @BukauLab]

Congratulations to our colleagues @ZmbhH for the great work!!

[Stefan Pfeffer @PfefferCryoLab]

How do you synthesize proteins with only half a ribosome? We are excited to share our work on the structural basis of Alanine tailing in bacterial ribosome-associated quality control (RQC). Great collaboration with @LabJoazeiro @ZmbhH.

https://t.co/LERrasmFtS

[Rosenzweig Lab @lab_rosenzweig]

PNAS selected our joint work with @BukauLab on the mechanism of fiber disaggregation to be highlighted on their journal club blog! @PNASNews

https://t.co/kK1866YRHg