Inga Songailiene

[Inga Songailiene @IngaTrails]

Things are moving to Blue sky!
https://t.co/it6sBTY8Wf

[Tominaga K. (tomiken) @pacyc1841]

Genome delivery of a contractile tailed phage and its superinfection exclusion mechanism | bioRxiv https://t.co/SJz13EHuC4

[Inga Songailiene @IngaTrails]

@crypticprophage No, we cited your work in ApeA paper because we respect it.
Regarding the mechanism - we described tRNA 3'-end cleavage activity which your mentioned TA is not doing.

[Inga Songailiene @IngaTrails]

Toprim is different domain than HEPN. MNT can fit in here.
tRNA cleavage expriments from our MNT-HEPN study really helped for Eco2. The study:
https://t.co/lQIHL5mhwB

[Cryptic Prophage (phage 🧬🪤 in 🦠) @crypticprophage]

https://t.co/6N24W2bDPD
Retron Eco2, another derivative of TA system HEPN-MntA (doi: 10.1093/nar/gkaa855), works as a trimeric complex that is activated by T2 phage endonuclease DenB, which cleaves msDNA & leads to RNA degradation.

[Inga Songailiene @IngaTrails]

@crypticprophage what do you mean? Eco2 has nothing to do on AMPylation. We cited your paper on ApeA HEPN study, our study complements your findings in NAR and adds extra info about tRNA cleavage that was relevant to Eco2 paper.

[Inga Songailiene @IngaTrails]

@crypticprophage Toprim is different domain than HEPN. MNT can fit in here.
tRNA cleavage expriments from our MNT-HEPN study really helped for Eco2. The study: https://t.co/lQIHL5mhwB

[Inga Songailiene @IngaTrails]

Deoxydinucleotides can activate doughnut-shaped mutimeric HEPN ApeA nucleases for tRNA cleavage in the anticodon loop ✂️🛡🧬
Great work lead by Jonas, Giedrius and great team 💪🙌

[Jonas Juozapaitis @JJuozapaitis]

🧬 We discovered how bacteria detect phage infections by sensing fragments of their own degraded DNA.
Meet #ApeA – a #BacterialImmunity system activated by a novel signal - #Deoxydinucleotides.

Read our preprint: https://t.co/pFdWEQlPlj
A thread 🧵 (1/11)

[Nicholas M. I. Taylor Lab @NMITaylorLab]

Happy to share that our paper on the PlPVC1 eCIS is out in @NatureComms 🥳 Congratulations to first author Leyre Marín-Arraiza and all co-authors! #cryoEM @cryoEM_Papers @UCPH_CPR https://t.co/2qdQFxXEI8

[Inga Songailiene @IngaTrails]

Impressive work!

[Cryptic Prophage (phage 🧬🪤 in 🦠) @crypticprophage]

https://t.co/4sBHqVXvD8
New type IX toxin/antitoxin system, anti-phage system Clover, where antitoxin produces 5′-triphosphothymidyl-3′5′-thymidine to inhibit the dGTPase toxin.

[Inga Songailiene @IngaTrails]

Impressive spacer selection mechanism by Cas9-adaptation supercomplexes - all pathway captured by #cryoEM #Cas9 🧬🧩🧵

[Giedrius Sasnauskas @GiedriusSasn]

'Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation'
Check out our preprint on #bioRxiv on the #Cas9-Cas1-Cas2-Csn2 supercomplex involved in #CRISPR spacer acquisition: https://t.co/8kVnCUKg1p
Thread below.

[Victor Klein-Sousa @VKleinSousa]

Excited to see our work published in @ScienceAdvances!

We present RBPseg, a method for structural prediction of #phage receptor-binding proteins.

We applied it to diverse tail fibers and validated the predicted structures using #cryoEM.

Read here: https://t.co/4tnb65svpY

[Avigail Stokar-Avihail @AAvihail]

I highly recommend attending this amazing microbiology conference!
🦠🔬👩🏼‍🔬🧬🧪

Only a few more days to submit your abstracts! (April 1st ).

Looking forwards! 🍻

[Shai Zilberzwige-Tal @zilberzwige]

RNA-guided systems span kingdoms, from bacterial immunity to eukaryotic gene regulation. How did they evolve? We traced the origins of CRISPR-Cas13 and identified its likely ancestor: an RNA-based toxin-antitoxin (TA) system. Here’s what we uncovered:
https://t.co/oTGjiN2V3H
1/8

[Vasili Hauryliuk @hauryliuk]

Looking forward to this.
https://t.co/AiRcXQ219j

[Inga Songailiene @IngaTrails]

I can add "Life's Engines: How Microbes Made Earth Habitable" by Paul G. Falkowski.
What more can you recommend for next year?

[Eric Topol @EricTopol]

My favorite books, 2024
by @emollick @firstadopter @anilananth @harari_yuval @carlzimmer and other great authors not on X

[Inga Songailiene @IngaTrails]

@TaraBartolec @KMitosch @savitski_lab @TypasLab Thank you for testing MNT-HEPN, ApeA and including them into the study! The mechanisms of activation for these systems still remain mysterious.

[Arturo Carabias @ArturoCarabias]

Happy to share our spotlight (with @g1_guillermo at @UCPH_CPR) in @CellChemBiol, highlighting the groundbreaking work by @IlyaOsterman & @SorekLab on how #phages reconstitute NAD to bypass bacterial immunity🦠🛡️. Check it out here! https://t.co/2Go6aExRvP 🤓

[Rafa Pinilla-Redondo @rafapinilla92]

Excited to share our latest collab with Wenyuan Han's Lab! 🚨 Bacteria use dITP as an immune signal to activate NAD+-depleting effectors. Phages counter immunity by destroying dAMP (dITP precursor)! 💥🧬
Preprint 🔗: https://t.co/cuGBzgc9mK

[Inga Songailiene @IngaTrails]

@pentamorfico @rafapinilla92 @leighton_payne 🤣

[haidai HU @HaidaiHu]

Thrilled to share that our Zorya paper is now published online in Nature! It was an incredible team effort @__jphilipp @Salmo_Lab @SimonJacksonNZ —thank you to everyone who contributed to this work.@IngaTrails @VKleinSousa @AritzRoa @freddie__martin @yongwangKU @AHarms485 @others