John Chorba

Chemical Biologist. Cardiologist. Dad. Solving clinical problems and improving human health through science.

[John Chorba @JChorba]

In @NatureCVR, I share suggestions to bring the best of @NIH to #academia. When we work together to fulfill commitments, we can be better scientists and public servants. Universities should give #earlycareer and #physicianscientist a level playing field! https://t.co/6HKb74dxR6

[John Chorba @JChorba]

Excited to share our protocol (https://t.co/z0S8woqkEX) in @bioprotocolbyte to rapidly screen through CRISPR hits! Thanks to Vivian Xia, Dr. Arun Padmanabhan, and @gsmithMDPHD and all those @UCSF who "validated" it for us!

[Bio-protocol @bioprotocolbyte]

New in #Genomics:🧬
Rapid multiplexed flow cytometric validation of #CRISPRi sgRNAs in tissue culture
Full protocol: https://t.co/mP6aJCYGVY

👏@JChorba and colleagues

[John Chorba @JChorba]

@keely_oltion @UCSF Loved the paper! It's so rare to get a paper that you can read like a novel (where the words are as clear as the figures)!

[John Chorba @JChorba]

Have to practice what you preach!

[Nia Beckley @sadebecks]

When you're back on cardiology at Zuckerberg General 1 yr later as a PGY2 w/ this incredibly diverse, awesome rock star team! ❤️❤️ Shout out to Dr. Chorba who encourages heart healthy exercise w/ 6 flights of stairs daily! 😅💪🏾😁 @UCSFIMChiefs #zsfg

[AnnMarieSchmidt_NYU @AMSchmidt_NYU]

In the most recent issue of ATVB, Xia and colleagues report on the relationship between heparin and plasma levels of PCSK9 - data from humans! Learn about the nuances between findings in animals vs the human in ATVB!

[ATVB: An AHA Journal @atvbahajournals]

Can heparin alter plasma PCSK9 levels? Animals studies suggest yes, but human data from @JChorba lab makes us think twice https://t.co/4gJIGHklee

[John Chorba @JChorba]

(4/4): Great work from a fantastic team: Dr (to be) Vivian Xia and the cath and clinical labs @UCSFCardiology and @ZSFGCare (@LucasZierMD, Bee Ong, and Drs. John MacGregor and Alan Wu).

[John Chorba @JChorba]

(1/4): Excited to share our lab’s latest tweetorial! Now out in @atvbahajournals (https://t.co/QUtK7at4TO), we asked an important clinical question: can the binding between #PCSK9 and heparin be repurposed to use heparin as a cheap PCSK9 inhibitor and lower #cholesterol?

[John Chorba @JChorba]

(3/4): This was true #frugalscience: we simply used remnant blood samples from routine care (which would otherwise be discarded!), so we could study the outcomes in the ideal physiologic system: real humans! And #peerreview (thanks reviewer #2!) led to our more nuanced answer.

[John Chorba @JChorba]

Great talk David! Inspiring to see work going all the way from algorithm development to actual clinical implementation!

[David Ouyang, MD @David_Ouyang]

Excited to hang out with old and new friends and talk at my alma mater! @UCSF @JamesETooley @jpirruccello @GeoffTison @arnaout_rima @yuanneal @JChorba @ucsf_echo @UCSFCardiology

[Science Translational Medicine @ScienceTM]

Today is #WorldCRISPRDay—ICYMI, the @JChorba lab at @UCSF recently leveraged #CRISPR screening to show the protein CSDE1 is a key regulator of the LDL cholesterol receptor, and could offer a feasible target for new #HeartDisease therapies. https://t.co/hlghcuzC29

[John Chorba @JChorba]

Blown away by the story of @ElectrosomeUCSF and @ZaroLab on the structural basis for “chaperone” assisted quaternary complex formation of ion channels. Paradigm-changing, yet makes sense! Is this druggable? Do we drug it now and just don’t know it? (https://t.co/UIsFd6jkCA)

[John Chorba @JChorba]

Congrats to @BrianZou_1 and @denniscao on their new method to make some useful chemical building blocks! (https://t.co/1ESmguK7YH)

[Raj Gupta @Dr_RajatGupta]

Loved this paper from @JChorba @gsmithMDPHD @pnatarajanmd. Such a well written example of a CRISPRi screen, with careful validation of the positive hits. Candidate genes are prioritized based on specificity and GWAS overlap.

https://t.co/pNcLY6b2hY

[John Chorba @JChorba]

Congrats to my @UCSF colleagues @DrEmilyGoldberg and @JChristophUCSF on being recognized for the research rock stars they are! (https://t.co/wNjZVYllCK)

[John Chorba @JChorba]

Love the collaborative effort here!

[Pradeep Natarajan @pnatarajanmd]

Nice summary of our collaborative work led by @gsmithMDPHD @JChorba discovering CSDE1 as a novel regulator of LDLR in @NatRevCardiol https://t.co/G4VtyKvmC3
(https://t.co/wte5I4sz3B @ScienceTM)

[John Chorba @JChorba]

Great summary of some of the key points of our work! Looking forward to chapter 2, and continuing these great collaborations!

[Nature Reviews Cardiology @NatRevCardiol]

#ResearchHighlight: CSDE1 identified as a novel post-transcriptional regulator of LDL receptor levels in the liver
https://t.co/mCp9JZSAK3

@JChorba @gsmithMDPHD @pnatarajanmd @kevanSF @dsrivastava_md @RolandSWu @tomohironn @akhilp2301 @BrianBlackUCSF
@UCSFCardiology

[John Chorba @JChorba]

Excited to pursue this further! We're always looking for enthusiastic scientists to join us, so reach out if interested! (https://t.co/hUKpjPziAR)

[Science Translational Medicine @ScienceTM]

A #CRISPR based screening study reveals that the protein CSDE1 is a key regulator of the LDL cholesterol receptor and could offer a feasible target for new #HeartDisease therapies, according to new experiments from the @JChorba lab at @UCSF. https://t.co/6US6wsquHY