Nowakowski_Lab

Developmental neurobiologist, using integrative high throughput technologies to study how the cerebral cortex is constructed.

[Nowakowski_Lab @LabNowakowski]

Excited to share our review of brain development, focusing on what is known about the emergence of cortical areas. Great work by @crcadwell @mohammedmostajo , Aparna Bhaduri and Matt Keefe https://t.co/hNK9L747fN

[Guille Lopez-Bendito @GuilleLBendito]

🚀 New from the lab! How do sensory cortices "know" whether they’ll become visual or somatosensory?🧠 We uncover early modality-identity genes in cortex, some set before thalamic innervation via cell-adhesion codes, others refined by thalamic activity. @GLB_Lab @NeuroAlc

[USBrainAlliance @USBrainAlliance]

Scientists complete first drafts of developing mammalian brain cell atlases.
Learn more: https://t.co/vRvqyLcySw #studyBRAIN

[Nowakowski_Lab @LabNowakowski]

@RongFan8 @Nature @GoCasteloBranco @Di_0579 @YaleEngineering @karolinskainst @YaleMed @UCSF @ASTARsg Congratulations on this amazing work!

[Sergiu P. Pasca @Sergiu_P_Pasca]

Less than two weeks left to register for the inaugural conference on Assembloids & Complex Cell-Cell Interactions @CSHL

Check out the topics and amazing lineup of speakers! 👇

[Nowakowski_Lab @LabNowakowski]

What an exciting time for brain mapping 👇🏻👇🏻👇🏻

[Andrew Payne @Andrew_C_Payne]

@E11BIO is excited to unveil PRISM technology for mapping brain wiring with simple light microscopes. Today, brain mapping in humans and other mammals is bottlenecked by accurate neuron tracing. PRISM uses molecular ID codes and AI to help neurons trace themselves. We discovered a new cell barcoding approach exceeding comparable methods by more than 750x. This is the heart of PRISM. We integrated this capability with microscopy and AI image analysis to automatically trace neurons at high resolution and annotate them with molecular features.

This is a key advance towards economically viable brain mapping - 95% of costs stem from neuron tracing. It is also an important step towards democratizing neuron tracing for everyday neuroscience. Solving these problems is critical for curing brain disorders, building safer and human-like AI, and even simulating brain function.

In our first pilot study, we acquired a unique dataset in mouse hippocampus. Barcodes improved the accuracy of tracing genetically labelled neurons by 8x – with a clear path to 100x or more. They also permit tracing across spatial gaps – essential for mitigating tissue section loss in whole-brain scaling. Using molecular annotation, we uncover an intriguing feature of synaptic organization, demonstrating how PRISM can be used for systematic discovery 🧵

[Guille Lopez-Bendito @GuilleLBendito]

📢 We're hiring a PhD student! Join the
@GLB_Lab (IN-UMH/CSIC; @NeuroAlc) to explore how spontaneous neural activity and genetic programs shape sensory cortical development. Funded by @AgEInves. https://t.co/VLbqCRKo0r

[Andrew Payne @Andrew_C_Payne]

@AdamMarblestone and I explore how we may design safer AI systems and improve brain health by applying new brain mapping technologies -- including new microscopy and AI tools from @E11BIO. Also check out the rest of this essay series on emerging technologies hosted by @IFP 👇

[Nowakowski_Lab @LabNowakowski]

@BrainPrize @michelle_monje @StanfordMed @HHMINEWS Congratulations to both awardees, very exciting time for Neuroscience!

[Nowakowski_Lab @LabNowakowski]

Congratulations Dena and team!!!

[Dena Dubal @DenaDubal]

"We could all use a little more X!" Thrilled our new study is out. Aging "wakes up" the silent X chromosome in the female brain - and this contributes cognitive resilience. Spearheaded by @MargaretGadek in @ScienceAdvances Collaborators Berenice Benayoun, @BBParis1984 Kaitlin Casaletto @YokoyamaLabUCSF @SimonsFdn @NIH https://t.co/Fko9tGDs1n

[Nowakowski_Lab @LabNowakowski]

@GoCasteloBranco @karolinskainst @HjerlingLeffler Congratulations Jens!!! 🎉

[Rongxin Fang, Ph.D. @RongxinFang]

We are looking for talented postdocs to join our team at @StanfordBrain @StanfordMed @StanfordNsurg! Join us to develop single cell sequencing- and imaging-based genomic tools to understand the cell communication across space and time! Please share with anyone interested!!

[Willow Coyote-Maestas @willowcoyote]

We are accepting applications for speakers from Native American trainees - if you know of someone who would be a good fit, encourage them to apply here: https://t.co/0mDbkA1Msc
PS: We have travel funds available! Plz share!
More info in the thread.

[Nowakowski_Lab @LabNowakowski]

Super cool science from my colleagues at UCSF- mutational scanning uncovers mechanisms of pH sensing by GPCRs

[Willow Coyote-Maestas @willowcoyote]

Check out our work, where we discover how receptor GPCR senses pH! We develop foundational GPCR DMS tech dev + structural bio that will have impact across historically intractable receptor systems! Led by brilliant PhD student @matthewkhoward and Nick Hoppe w/ @AashishManglik!

[Nowakowski_Lab @LabNowakowski]

👇🏻👇🏻👇🏻

[Arpiar Saunders, PhD @your_arpy]

Our latest work in collaboration with the Bourhy Lab (Pasteur Inst.) in which we use scRNA-seq to characterize host-rabies molecular interactions across diverse human brain cell types. Recommended if you're interested in "system virology" or glial biology: https://t.co/jPhuG3z3SL

[Nowakowski_Lab @LabNowakowski]

👇🏻👇🏻👇🏻

[Ozgun Gokce @ozgungokce]

Spotlight on Single-Cell Genomics!
We (@ozgungokce , @bayraktar_lab, @naomi_habib, Genevieve Konopka, @LiddelowSA , and @LabNowakowski) collaborating with top neuroscientists to explore optimizing these technologies:
🔗 https://t.co/IRb1O6TM71

[Nowakowski_Lab @LabNowakowski]

👇🏻👇🏻👇🏻

[Shan Meltzer @shanmeltzer]

We are looking for a postdoctoral fellow to join us to study somatosensation development and function! I’m committed to the training and success of my trainees. Please help me spread the words!

[Nowakowski_Lab @LabNowakowski]

👇🏻

[John @JohnPAndrews_MD]

4/14 We used human hippocampus from patients who had brain surgery for refractory epilepsy. We sliced samples and transduced them on day 0, and our custom tissue-prep pipeline allowed for our high-density MEA recording experiments after up to 8 days in tissue culture.

[Nowakowski_Lab @LabNowakowski]

Excited to share new study from the lab, work led by @JohnPAndrews_MD and grateful for all the collaborators who made this possible including @ChangLabUcsf @ucscgenomics

[UCSF Neurosurgery @NeurosurgUCSF]

Today in @NatureNeuro, researchers led by @LabNowakowski, @ChangLabUCSF & Mircea Teodorescu (@ucscgenomics) demonstrate the first use of optogenetics to modulate seizure activity at the network level in human brain tissue:
https://t.co/GquVhx1axa
@JohnPAndrews_MD @UCSFstemcell

[Chongyuan Luo @chongyuanluo]

Glad to share the peer-reviewed version of the @mgheffel manuscript now in @Nature. In collaboration with @lab_paredes , we studied the chromatin conformation and DNA methylation changes during human hippocampus and frontal cortex development. https://t.co/T8idzapvf5