Scott James

CAR T cell researcher at City Of Hope

[Scott James @ScottJamesCART]

Happy to share our new study in @natBME describing our “Zip-sort” strategy to purify dual-transduced cells with larger transgene payloads. Thanks to all our collaborators. @Parkerici @Cityofhope @MSKCancerCenter @Bushmanlab @VardhanaLab @KlebanoffLab https://t.co/kxk0d6cgm2. 1/

[Kyle G Daniels @ScienceKyle]

🚨What if we could reliably program macrophage polarization state?🚨

https://t.co/6qGxuHptQq

Macrophages are highly plastic immune cells that perform critical functions by polarizing into distinct cellular states. The polarization state of macrophages can substantially influences the progression of cancers, infections, and autoimmunity. (1/11)

[Ansu Satpathy @Satpathology]

Excited to introduce CRISPR-All! – a flexible, high-throughput framework that combines virtually any perturbation modality (CRISPRko, CRISPRi, CRISPRa, shRNA, knock-in, base/prime editing, etc.) in the same cell, at scale.

Paired with single-cell RNA-seq @10xGenomics and rich phenotypic readouts, it unlocks massively combinatorial genetic screens and new cell states previously out of reach.

Outstanding work led by @Roth_Lab @AustinMHartman

https://t.co/hU3OKDfMSd

[Majzner_Lab @Majzner_Lab]

Congrats to @mcrotiroti on her recent paper published in Nature Cancer now with an accompanying Research Briefing published today: https://t.co/8oxXLaijLU... Mari utilized insights into T cell proximal signaling to overcome antigen-low escape. I have been overdue to write a 🧵!

[Klebanoff_Lab @KlebanoffLab]

🚨Today, in @NatureCancer, we show that CAR-T cells and CAR-NK cells contain the seeds of their own self-destruction. Disruption of a FAS-L/FAS circuit enhances CAR potency against liquid and solid cancers. https://t.co/afS9vRG4Ok @MSKCancerCenter @parkerici @MSK_DeptOfMed

[Roni Shouval @RShouval]

🚨 Just out @NatureMedicine!
Introducing InflaMix—a point-of-care tool that identifies an #inflammatory 🔥 signature predictive of #CART failure in #lymphoma
Led by Sandeep Raj (@SSRaj017) 👏@MSKCancerCenter
https://t.co/k0tQMZBSxS 🧵Let’s break it down

[Klebanoff_Lab @KlebanoffLab]

🚨Today in @Nature, we show that mRNA mis-splicing creates clonal public neoantigens in solid cancers. An amazing collaboration between @UCSFCancer and @MSKCancerCenter driven by @DarwinKwok1 and co-led by Hideho Okada, @josephcostell14 and @KlebanoffLab. https://t.co/lDEGbQr7cT

[Justin Eyquem @j_eyquem]

🚨New paper alert! 🚨 Our latest work in @ImmunityCP shows that Ark313, an evolved AAV variant enables in vivo genetic engineering of murine T cells! 🧵👇

https://t.co/CkHpDdPy7I

[Nature Medicine @NatureMedicine]

In an analysis of long term safety events in 783 patients treated with #Tcell therapy in 38 trials, 2.3% of patients developed second primary malignancies, and vector integration analyses revealed no pathological insertions
https://t.co/ph8DH0tFdx

[van den Brink Lab @vandenBrinkLab]

The cover for our @BloodJournal article  “IL-18-secreting multiantigen targeting CAR T cells eliminate antigen-low myeloma in an immunocompetent mouse model” was selected as a finalist in the 2024 Blood Cover Contest! Please vote for us at https://t.co/fDYXD4JqW7 !

[Evan Weber @EvanWeberPhD]

Really cool to see a CAR T cell-focused exhibit at @TheFranklin Institute in Philly! @PennMedicine @CHOP_Research @carlhjune

[Scott James @ScottJamesCART]

In summary, our proof of principle study demonstrates that Zip-sorted T cells co-expressing multiple CARs and switch receptors can eliminate tumor populations with high target antigen diversity while resisting exhaustion and inhibitory ligand-mediated suppression. 14/

[Scott James @ScottJamesCART]

Happy to share our new study in @natBME describing our “Zip-sort” strategy to purify dual-transduced cells with larger transgene payloads. Thanks to all our collaborators. @Parkerici @Cityofhope @MSKCancerCenter @Bushmanlab @VardhanaLab @KlebanoffLab https://t.co/kxk0d6cgm2. 1/

[Scott James @ScottJamesCART]

However, co-expression of switch receptors promoted T cell expansion in vivo and improved anti-leukemia activity of quad-CAR T cells. 13/