Amarelowbr

Health research, emphasis on vitamin D. Currently studying Alzheimer's disease and dementia. https://t.co/LzPpCtWGuv. https://t.co/Jl5a55EB5h

1 day ago

@CharlesMBrenner What is your opinion on nicotinamide riboside tartrate (NRT)? Could it work as well as Niagen?

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amarelowbr retweeted

Charles Brenner, PhD

@CharlesMBrenner

4 months ago

fun fact: same PI, same trial design tested metformin and NR to improve walking speed in peripheral artery dz metformin failed https://t.co/BiZXlYdSPA NR succeeded https://t.co/3GoY2JcMKO

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amarelowbr retweeted

Charles Brenner, PhD

@CharlesMBrenner

21 days ago

some things you probably don't know about NAD and blood: a thread

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Who to follow

William B. Grant, PhD

@wbgrant2

TexasLindsay™

@TexasLindsay_

JMFF Fellow | Free Speech Cognoscente | See Highlights Tab for #TwitterFiles Coverage | Auburn University Alum | War Eagle 🦅🇺🇸

Dr Joffre Rodríguez Sarmiento

@dr_joffre

Cardiólogo Clínico - Ecocardiografista - Ecoestrés - Eco Transesofágico / Ecuatoriano - Especializado en Buenos Aires - Argentina

amarelowbr retweeted

Bryan Johnson

@bryan_johnson

about 3 years ago

NR vs NMN? I used each for 90 days & both were effective in getting my intracellular NAD levels to age 16 equivalent, 31 yr age reversal as semi-indirect bioage marker. Recent levels 54.6μM via Jinfiniti test. Used @truniagen NR & @JinfinitiPM NMN.

bryan_johnson's tweet photo. NR vs NMN?

I used each for 90 days & both were effective in getting my intracellular NAD levels to age 16 equivalent, 31 yr age reversal as semi-indirect bioage marker. Recent levels 54.6μM via Jinfiniti test. Used @truniagen NR & @JinfinitiPM NMN. https://t.co/YRoSkoO3cM

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amarelowbr retweeted

8 months ago

Nicotinamide Riboside (vitamin B3) extends lifespan. Here it was shown to: ◈ Upregulate protective gene expression (mitochondrial metabolism, DNA repair) ◈ Downregulate inflammatory gene expression ◈ Improve stem cell health + function (drop in aging, lower regenerative capacity) NAD+ / NADH ratio drops with age - optimizing it is one of the keys to a healthy + long life.

Outdoctrination's tweet photo. Nicotinamide Riboside (vitamin B3) extends lifespan.

Here it was shown to:

◈ Upregulate protective gene expression (mitochondrial metabolism, DNA repair)
◈ Downregulate inflammatory gene expression
◈ Improve stem cell health + function (drop in aging, lower regenerative capacity)

NAD+ / NADH ratio drops with age - optimizing it is one of the keys to a healthy + long life.

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amarelowbr retweeted

오창명

@changmyung1981

5 days ago

NCoR1: A Master Regulator of Intestinal Aging Reversed by Metformin Aging doesn't just affect muscles, brains, and blood vessels—it reshapes the gut at the cellular level. A new Nature Aging study generated a single-nucleus atlas of the aging primate small intestine and identified NCoR1 (Nuclear Receptor Corepressor 1) as a central regulator of intestinal aging that can be restored by long-term metformin treatment. Researchers profiled 64,558 nuclei from young and aged cynomolgus monkey small intestines and uncovered several hallmarks of intestinal aging: 🔹 Reduced villus height and crypt depth 🔹 Increased epithelial senescence 🔹 Barrier dysfunction with loss of ZO-1 and E-cadherin 🔹 Chronic inflammation and oxidative stress 🔹 Increased retrotransposon activation 🔹 Reduced intestinal stem-cell function 🔹 Differentiation bias away from absorptive enterocytes toward secretory lineages such as Paneth cells Single-nucleus transcriptomics revealed widespread activation of inflammatory programs including NF-κB signaling, cytokine responses, SASP, oxidative stress, and apoptosis, while genes involved in nutrient absorption and intestinal homeostasis declined. Among all aging-associated regulators, NCOR1 emerged as one of the most consistently downregulated genes across epithelial, immune, and stromal compartments. Importantly, this decline was conserved in human intestinal datasets and human biopsy samples. Functional validation demonstrated causality: ✅ NCOR1 knockdown in human intestinal epithelial cells induced: • SA-β-gal positivity • DNA damage • p21 activation • Junctional disruption • Reduced proliferation • Increased inflammatory signaling ✅ Human intestinal organoids with NCOR1 depletion developed: • Stem-cell exhaustion • Reduced growth • Expansion of Paneth cells • Features resembling aged intestine The most striking result came from intervention studies. Long-term metformin treatment for 40 months in aged monkeys restored intestinal NCoR1 levels, reduced inflammation, improved epithelial proliferation, and delayed biological intestinal aging measured by a single-cell transcriptomic aging clock. The average intestinal biological age was reduced by approximately 1.17 years, with some epithelial populations showing even larger rejuvenation effects. Mechanistically, metformin's anti-aging effects were largely lost when NCOR1 was suppressed, placing NCoR1 directly downstream of metformin action. This study positions the aging intestine as a previously underappreciated target of geroscience interventions and identifies NCoR1 as a pharmacologically actionable hub linking stem-cell maintenance, barrier integrity, inflammation, and epithelial homeostasis. The implication is clear: Maintaining NCoR1 may be a key strategy for preserving gut function, nutrient absorption, immune resilience, and systemic health during aging. Reference Li J, Lu X, Tong T, et al. Single-nucleus interrogation of primate small intestinal aging reveals NCoR1 decline as a conserved feature that is reversed by metformin. Nature Aging (2026) DOI: 10.1038/s43587-026-01131-0 #Aging #GutAging #NCoR1 #Metformin #Geroscience #SingleCell #Organoids #StemCells #Longevity #NatureAging

changmyung1981's tweet photo. NCoR1: A Master Regulator of Intestinal Aging Reversed by Metformin

Aging doesn't just affect muscles, brains, and blood vessels—it reshapes the gut at the cellular level.

A new Nature Aging study generated a single-nucleus atlas of the aging primate small intestine and identified NCoR1 (Nuclear Receptor Corepressor 1) as a central regulator of intestinal aging that can be restored by long-term metformin treatment.

Researchers profiled 64,558 nuclei from young and aged cynomolgus monkey small intestines and uncovered several hallmarks of intestinal aging:

🔹 Reduced villus height and crypt depth

🔹 Increased epithelial senescence

🔹 Barrier dysfunction with loss of ZO-1 and E-cadherin

🔹 Chronic inflammation and oxidative stress

🔹 Increased retrotransposon activation

🔹 Reduced intestinal stem-cell function

🔹 Differentiation bias away from absorptive enterocytes toward secretory lineages such as Paneth cells

Single-nucleus transcriptomics revealed widespread activation of inflammatory programs including NF-κB signaling, cytokine responses, SASP, oxidative stress, and apoptosis, while genes involved in nutrient absorption and intestinal homeostasis declined.

Among all aging-associated regulators, NCOR1 emerged as one of the most consistently downregulated genes across epithelial, immune, and stromal compartments. Importantly, this decline was conserved in human intestinal datasets and human biopsy samples.

Functional validation demonstrated causality:

✅ NCOR1 knockdown in human intestinal epithelial cells induced:

• SA-β-gal positivity

• DNA damage

• p21 activation

• Junctional disruption

• Reduced proliferation

• Increased inflammatory signaling

✅ Human intestinal organoids with NCOR1 depletion developed:

• Stem-cell exhaustion

• Reduced growth

• Expansion of Paneth cells

• Features resembling aged intestine

The most striking result came from intervention studies.

Long-term metformin treatment for 40 months in aged monkeys restored intestinal NCoR1 levels, reduced inflammation, improved epithelial proliferation, and delayed biological intestinal aging measured by a single-cell transcriptomic aging clock. The average intestinal biological age was reduced by approximately 1.17 years, with some epithelial populations showing even larger rejuvenation effects.

Mechanistically, metformin's anti-aging effects were largely lost when NCOR1 was suppressed, placing NCoR1 directly downstream of metformin action.

This study positions the aging intestine as a previously underappreciated target of geroscience interventions and identifies NCoR1 as a pharmacologically actionable hub linking stem-cell maintenance, barrier integrity, inflammation, and epithelial homeostasis.

The implication is clear:

Maintaining NCoR1 may be a key strategy for preserving gut function, nutrient absorption, immune resilience, and systemic health during aging.

Reference

Li J, Lu X, Tong T, et al.

Single-nucleus interrogation of primate small intestinal aging reveals NCoR1 decline as a conserved feature that is reversed by metformin.

Nature Aging (2026)

DOI: 10.1038/s43587-026-01131-0

#Aging #GutAging #NCoR1 #Metformin #Geroscience #SingleCell #Organoids #StemCells #Longevity #NatureAging

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amarelowbr retweeted

5 days ago

2 Kiwis a day reverses signs of skin aging in clinical trial. The kiwis increased skin density by ~50% after 8 weeks. The green here represents functional tissue, mainly collagen protein, in the skin. Kiwis also improved the rate of skin cell proliferation - A loss of this process means the skin is aging and deteriorating. This is due to kiwis' sky high content of vitamin C.

Outdoctrination's tweet photo. 2 Kiwis a day reverses signs of skin aging in clinical trial.

The kiwis increased skin density by ~50% after 8 weeks.

The green here represents functional tissue, mainly collagen protein, in the skin.

Kiwis also improved the rate of skin cell proliferation - A loss of this process means the skin is aging and deteriorating.

This is due to kiwis' sky high content of vitamin C.

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amarelowbr retweeted

7 days ago

If you want to live longer, read this. These are the 7 KEYS TO EXTENDING LIFESPAN in research (🧵1/8):

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amarelowbr retweeted

Agingdoc🩺Dr David Barzilai🔔MD PhD MS MBA DipABLM

9 months ago

#4 Low NAD+ NAD+ levels have been shown to decline almost linearly with age in key tissues. Here is the correlation in the cerebral cortex of the brain. (7/10)

Outdoctrination's tweet photo. #4 Low NAD+

NAD+ levels have been shown to decline almost linearly with age in key tissues.

Here is the correlation in the cerebral cortex of the brain.

(7/10) https://t.co/qgZDSPgOxl

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amarelowbr retweeted

@agingdoc1

over 3 years ago

Nicotinamide riboside improves muscle mitochondrial biogenesis, satellite cell differentiation, and gut microbiota in a twin study @DrPietilainen, @pirinenlab & coauthors https://t.co/f6ohk9NwoQ @ScienceAdvances

agingdoc1's tweet photo. Nicotinamide riboside improves muscle mitochondrial biogenesis, satellite cell differentiation, and gut microbiota in a twin study

@DrPietilainen, @pirinenlab & coauthors

https://t.co/f6ohk9NwoQ

@ScienceAdvances https://t.co/gaXVTnVbr7

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30 days ago

@HarrySpoelstra L-arginine + Vitamin C to restore nitric oxide production and endothelial function

9 months ago

Combining L-Arginine with vitamin C improves long-COVID symptoms: The LINCOLN Survey https://t.co/oDMCgBeUJ7 #larginine #arginine #covid #longcovid #arginina #CovidLonga #pandemia #tratamentoprecoce #saude #fadiga

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about 1 month ago

@dlingenfelter L-arginine + Vitamin C

9 months ago

Combining L-Arginine with vitamin C improves long-COVID symptoms: The LINCOLN Survey https://t.co/oDMCgBeUJ7 #larginine #arginine #covid #longcovid #arginina #CovidLonga #pandemia #tratamentoprecoce #saude #fadiga

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about 1 month ago

@NicHulscher The Argentine government inaugurated a level 4 laboratory that studies Hantavirus in October 2025. https://t.co/qcR2GFeV07

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about 2 months ago

Longitudinal associations between micronutrient consumption and leukocyte telomere length https://t.co/dTY9AySniY

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