Dylan Rausch

CPH Bioscience PhD student with @tunepers. Studying CNS regulation of metabolism with single-cell 🧠 Crossfit 🏋️‍♀️ fantasy nerd 📚 Red Sox ⚾️

[Dylan Rausch @dylanrausch]

@jackmac M

[Tune H. Pers @tunepers]

New preprint on a cross-species atlas of the dorsal vagal complex and neural mediators of cagrilintide’s effects on energy balance @metabolcenter
>580,000 cells mapping to 89 rat, mice and rhesus macaque cell populations!

https://t.co/jaoqRk0q19

#Neuroscience #Obesity
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[Dylan Rausch @dylanrausch]

@GESTALT_sp @10xGenomics I'm working with postxenium IF imgs that I've aligned in xeniumexplorer. Is there a way to apply the transformation matrix to the postxenium img so I can load the img and xenium ROIs into qupath? I want to use a classifier to identify +/- cells!

[Michael Totty @MicTott]

I'm very excited to share my first postdoc preprint with
@BoyiGuo and @stephaniehicks!

Here we preset SpotSweeper - the first spatially-aware quality control methods developed for spatial transcriptomics. 🧵
https://t.co/3ak7ZxCRHF

[Dylan Rausch @dylanrausch]

@CalebLareau @tsion_abay @MerilTakizawa @ChaligneRonan @Satpathology Perfect, love the idea of a dirty sort. This is gonna be a game changer for neuroscience for sure. Can’t wait to try sorting out activated neurons without viral strategies or genetic mouse models

[Nature Communications @NatureComms]

Fantastic new article from the @ClemmensenC lab! Here, the authors show that the compensatory response that counteracts weight gain and overfeeding is preserved in #obesity. @Metabolcenter
https://t.co/RF8gMkwbp1

[Dylan Rausch @dylanrausch]

@the_tessallator This is super cool. Do you have any thoughts on adapting this approach to quantify the similarity between cell-types responding to a single perturbation (as opposed to the opposite shown here)?

[Dylan Rausch @dylanrausch]

@Matthew_N_B We've also been giving a lot of thought to this approach lately. I recently came across this paper which I think nicely handles binarized single cell data: https://t.co/qEyL5YM9f9

[Deniz Atasoy @AtasoyLab]

The time of day that we eat has significant impact on body weight and metabolic health but what determines those mealtimes? In a new study @NatureNeuro, we monitored daily oscillations in the activity of key hypothalamic hunger neurons expressing AgRP. ++ https://t.co/v9eBTt2Aqy

[Dylan Rausch @dylanrausch]

@ChenLiuLab @STARProtocols Great! Looking forward to the story. Have been wondering what cells we are actually labeling when crossing pomc-cre mice! I can imagine it will be an exciting one with this level of attention to detail!

[Dylan Rausch @dylanrausch]

@anshulkundaje @vitaliikl @neal_amin @drklly @tangming2005 @threadreaderapp unroll

[Daniel J Drucker @DanielJDrucker]

Conserved GABAergic LepRbGlp1r neuron population plays crucial roles in the suppression of food intake by leptin and GLP1R agonists @UMich #weightloss @seeleyrj @lotte_bjerre #obesity https://t.co/RZtTTzjU8M

[Dylan Rausch @dylanrausch]

@LiddelowSA @samuel_marsh @macosko @NaomiHabib2 @slinnarsson @LabNowakowski @ItaiYanai @tangming2005 I would also say if you want to enrich on the fluorescent marker with nuclei, we’ve been including a fluorphore fused to H2B (or sun1) in the AAV - very stable and clear positive populations for sorting in our hands

[Dylan Rausch @dylanrausch]

@LiddelowSA @samuel_marsh @macosko @NaomiHabib2 @slinnarsson @LabNowakowski @ItaiYanai @tangming2005 we can detect aav and/or rabies sequences though with pretty high sensitivity - including a primer specifically to amplify these reads in the cDNA amplification step of the 10x protocol really helps

[Dylan Rausch @dylanrausch]

@LiddelowSA @samuel_marsh @macosko @NaomiHabib2 @slinnarsson @LabNowakowski @ItaiYanai we have had terrible luck detecting gfp/tdtomato reads unfortunately... not sure why, could be just lowly transcribed or we don't have the exact correct sequences to align... @tangming2005 has a great post from a while ago where he saw the same issue: https://t.co/tGYYK447QY

[Florian T. Merkle PhD, Bluesky: @florianmerkle @FlorianMerkle]

We’re thrilled to share our preprint (collab with @marionilab) in which we use human POMC neurons to identify potential anti-obesity drugs, one of which seems to increase the efficacy of Semaglutide (Ozempic). Details on BioRxiv: https://t.co/lkdYYhFDq7. Thread below 🧵(1/16)

[Tune H. Pers @tunepers]

Great to have you in Copenhagen @Metabolcenter @SethBlackshaw and @ThomasDWKim 🙌 Inspiring talks from you and @Kirkeby_Lab on constructing and deconstructing the hypothalamus👌

[Rhiana C Simon, PhD @mouse_braind]

Super excited to finally share this preprint from a collaboration with Marta Soden here at UW! We combined optogenetics with snRNAseq to reveal input- and cell-type-specific activation patterns in the VTA 👇
https://t.co/hMMN0eCQbH