What if pooled screens could measure dynamic cell behavior in real time?
RainBar uses fluorescent protein barcodes and cytoplasmic marker mediated segmentation to perform optical pooled screening in live cells.
Preprint: https://t.co/BNfD9o7zg0
We are so happy to help Su Guo lab at UCSF use our new method LR-ExM to visualize Par-3 assisted polarized endosome dynamics in zebrafish. https://t.co/jrJ38eFbPj, @BoHuangLab, @IanSeiple . https://t.co/uESUd8vXU6
.@XiaoyuShi2, Li, @IanSeiple@BoHuangLab et al. report the development of label-retention expansion #microscopy w/ a set of trifunctional anchors that not only prevent fluorescent signal loss but also enable high-efficiency labeling using SNAP & CLIP tags. https://t.co/tdrksqXQAu
Just coming out in @CellCellPress: https://t.co/Xa7vmxTnit. Our incredible collaboration with the
@BivonaLabUCSF and @TulpuleLab shows how cytoplasmic protein granules formed by a variety of oncogenic kinase fusion mutants can activate RAS/MAPK signaling.
Endogenous tagging + 3D live-cell imaging + mass-spec interactome = https://t.co/83dtszwHs7, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann@loicaroyer & many other colleagues. @OpenCellCZB
Transfer learning solves the challenge of training data collection for supervised learning and also improves the performance of self-supervised learning. For image denoising, see bioRxiv https://t.co/KKZspCSFUc and GitHub https://t.co/H3JpwH9l5F
Still more to be done, but here is the 3rd-gen of green-colored split fluorescent protein for protein tagging and signal amplification https://t.co/aqosKepzZ6