Bug Slayers

@WellcomeTrust and @BBSRC funded projects studying the structure and function of the #Cdiff S-layer with @GillDouce1, @PerBullough, @PSSalgado and @RobFagan

[Bug Slayers @BugS_layers]

Bug Slayers is a collaborative effort involving 4 research groups, 4 postdoctoral scientists and 2 PhD students @SheffieldUni, @UofGlasgow & @UniofNewcastle In this thread I’ll introduce our team

[Bug Slayers @BugS_layers]

Fantastic to see another beautiful S-layer structure! Congratulations to the @RemautHan group
https://t.co/MABbcNQzzF

[Vincent Young @a2binny]

#ClostPath2023 comes to a close. What a fabulous meeting!!!

[Wiep Klaas Smits @SmitsLab]

And so we finish #clostpath2023, and look ahead to #clostpath2025 in #Paris! Local organisers @JohannPeltier @MorvanClaire2 and Thomas Candela. Good luck!

[Wiep Klaas Smits @SmitsLab]

Did I mention that besides great science #clostpath2023 also features stunning autumn weather? @ramelnyk held word!

[Dr Hannah Fisher @Hannah_Fisher2]

Really enjoyed sharing our work in Clostridium sporogenes at #Clostpath2023 this morning! Thanks to @dw_scientific for supporting my attendance at the conference and of course to the lab @PerBullough @RobFagan
@BeckyMenday @BuddleJess

[Bug Slayers @BugS_layers]

@RobFagan @GillDouce1 @pssalgado @MOrmsby8 @PaolaLanzoni @Joekaryotic We also determined another cool #BugSlayer structure, a tour de force from @barwinskasendra. Confirms our model for how SlpA packs into a lattice, with minor adjustments of charged interfaces.

[Bug Slayers @BugS_layers]

New paper from the @BugS_layers team! We show that the S-layer is necessary for virulence in mice and that the in vivo environment exerts a strong selective pressure that favours restoration of the S-layer in an S-layer-null mutant

https://t.co/Jj6B0SsMCw

[Bug Slayers @BugS_layers]

Required reading for all interested in S-layers from @TBharat_lab

https://t.co/uq1GbOHTly

[Thomas Seviour @Incottonwool]

Surface (S-) layer proteins are a bridge that connects multispecies microorganisms in anammox biofilms https://t.co/tkUSZ6vrRg #behindthepaper

[Bug Slayers @BugS_layers]

Always exciting to see a new S-layer structure! Beautiful work here from @DaumLaboratory

[Bertram Daum @DaumLaboratory]

What do archaeal cell surfaces look like?
Check out our latest preprint on the structure of the S-layer of the archaeon Sulfolobus acidocaldarius! https://t.co/KJ9ZOdYPjM!

[Bug Slayers @BugS_layers]

Next Monday @pssalgado will share our work on #Cdiff S-layer at the Cell Envelopes Symposium - register to join and please spread the word!
https://t.co/PTDfhNPutM

[Nature Communications @NatureComms]

Solving the C. difficile’s armour - while developing my own

@PaolaLanzoni tells her story #BehindThePaper

@BugS_layers @barwinskasendra @JSamWilson @OishikBanerji @PerBullough @RobFagan @pssalgado @GillDouce1 @FairweatherNeil @Joekaryotic @filipacostavaz
https://t.co/IfngFHJOQX

[Bug Slayers @BugS_layers]

Congratulations Shauna! The newest @BugS_layers PhD

[Bug Slayers @BugS_layers]

@matt_herdman Working on this and other aspects of S-layer 😉

[Bug Slayers @BugS_layers]

Our work revealing the structure and organisation of #BugSlayer in #Cdiff is finally out in @NatComms! Huge team effort from joint 1st authors @PaolaLanzoni, @barwinskasendra, @JSamWilson, @OishikBanerji! #SlayerStructure https://t.co/sHOiuGnOqs 1/n

[Bug Slayers @BugS_layers]

@ShenLabTufts @natcomms @PaolaLanzoni @barwinskasendra @JSamWilson @OishikBanerji Thank you Aimee! 😊

[Bug Slayers @BugS_layers]

@matt_herdman Thank you!

[Bug Slayers @BugS_layers]

Many new questions to answer: how do things go in/out of the #Cdiff cells? How can we disrupt this armour? How does the #SlayerStructure link to function and infection? Lots to keep us busy, but for now, a huge thank you to the whole @BugS_layers team! 11/11

[Bug Slayers @BugS_layers]

8. Removing the most exposed SLPL domain (D2), doesn’t change the overall SlpA structure and a functional S-layer is assembled. However, #Cdiff then becomes susceptible to lysozyme, even though the pores are still very narrow (16A). #SlayerStructure #BugSlayers 10/n

[Newcastle University @UniofNewcastle]

The spectacular structure of the protective armour of superbug #Cdiff revealed for the 1st time by @pssalgado (@MolMecNCL) @BugS_layers in @NatureComms shows the close-knit outer layer, like chain mail, protects the bug & provides a new target for drugs. https://t.co/DTdvJfCJYo