Victor Corces

[Victor Corces @CorcesVictor]

@Pete_Brahms @SecKennedy This article for Fauci is interesting in this context Rethinking next-generation vaccines for coronaviruses, influenzaviruses, and other respiratory viruses: Cell Host & Microbe

[Victor Corces @CorcesVictor]

@stutzman_alexis @DMcKay_Lab @jdOwen1 @DuronioLab Congratulations Alex, great news!!!!

[Victor Corces @CorcesVictor]

@Anders_S_Hansen @DomenicNarducci @deWitLab @hansen_lab @GRO_Broad @kochinstitute @MITdeptofBE It may be worth doing one of those old fashion westerns running CTCF on a gel and probing with the Znf143 antibody to see if it really cross-reacts. Just in case all the indirect evidence has an alternative explanation

[Victor Corces @CorcesVictor]

@cshperspectives It looks like they used an antibody from a different company than at least some of the previous papers. Even if it was from the same company, maybe previous batches didn't cross react. In previous manuscripts, only a subset of ZnF143 sites overlap with CTCF.

[Victor Corces @CorcesVictor]

@dphansti Well deserved!!

[Victor Corces @CorcesVictor]

@LugerLab Did you get these because it looks like DNA wrapping around octamers?

[Victor Corces @CorcesVictor]

@sr120 Sorry to hear. This is from a T-shirt celebrating his 50th birthday

[Victor Corces @CorcesVictor]

@AkisPapantonis Appropriate chips for the seminar

[Victor Corces @CorcesVictor]

This is a must-read paper for those interested in chromatin 3D organization. Compartments are way smaller than generally thought

[Jordan Rowley @MJordanRowley]

It's been a while since I posted. Pleased to announce 2 papers! Both about chromatin compartments.
Using deep sequencing in collaboration with ENCODE, we identify A and B compartments at 500 bp resolution! #3Dgenome @NatureComms
Out today: https://t.co/ibFfTXXe7j
See Thread.

[Victor Corces @CorcesVictor]

@minouye271 North, Central, or South America? If North, is it Canada, US, or Mexico?

[Victor Corces @CorcesVictor]

@StearnsLab @eLife At least you know it's going to be accepted and can list it as in press, right? 😊

[Victor Corces @CorcesVictor]

@dphansti You should change the title of the paper to "If you need to draw black lines to show people where the domains are, then you need to sequence more"

[Victor Corces @CorcesVictor]

Very important paper. Everyone planning to use Hi-C to compare changes of interactions between two conditions should read this
https://t.co/KkwZfzdRvw

[Victor Corces @CorcesVictor]

@doctorcorces

[Liam Satre Meloy @lsatremeloy]

My 3 year old daughter Lila was recently diagnosed with a VERY rare and aggressive type of #leukemia.

👉 It’s potentially treatment resistant… AND

👉 Requires a bone marrow transplant.

A🧵with all the details we have so far 👇

Please @Twitter help me save my little girl.

[Victor Corces @CorcesVictor]

We have an exciting program with amazing speakers and lots of room for talks selected from the abstracts!!!!!

[Mandy Meijer @mandy_meijer]

Only 3 weeks left to sign up for the #GenomeArchitecture #GRS if you would like to be considered for an oral presentation! We welcome all #PhD students and #Postdocs interested in #Chromatin biology! Deadline is March 5th! #GRC

[Victor Corces @CorcesVictor]

On the origin of the integrity of a subset of Topologically Associating Domains upon Cohesin loss in Interphase Chromosomes https://t.co/UYw8gTyCkg
More proof that there are two types of TADs forming by different mechanisms. Using "TAD" to refer to both of them is confusing.

[Victor Corces @CorcesVictor]

3/ The right way for you to show that the results are due to cfDNA is to purify 100,000 sperm, isolate the cfDNA from this prep, and then do ATAC-seq or Hi-C. What do you see? Probably nothing because there is not enough cfDNA

[Victor Corces @CorcesVictor]

1/ Of course we didn't use DTT. It makes no sense to try to analyze the state of chromatin by first destroying it with DTT. You are contaminating the sperm with DNA from the epididymis by mincing the tissue, which we don't do.

[Victor Corces @CorcesVictor]

2/ In the conditions we use, the nuclei are permeabilized based on movement of dyes into the nucleus. The DNA is extracted after ATAC-seq by proteinase K and phenol extraction, which you don't seem to do according to the manuscript.

[Victor Corces @CorcesVictor]

Maybe you are not purifying the sperm properly? We don't find any cell free DNA in sperm preps from the cause epididymis and no differences between treating or not with DNase. Also, no difference with ejaculated sperm after gradient purification