Nat B. Wang 🧬🧑🏻‍🔬🏳️‍🌈

PhD candidate @GallowayLabMIT interested in using synthetic and systems bio approaches to engineer cell fate transitions

[Nat B. Wang 🧬🧑🏻‍🔬🏳️‍🌈 @nbwang22]

1st preprint is finally out! When I started, as a 🤖 synbio person, there were so many Q's I had on how to build reprogramming vectors 🔧🧬. There's a lot more to do but we show how you encode reprogramming cocktail really matters in TF-specific ways! See tweetorial for more

[Katie Galloway @GallowayLabMIT]

How do individual transcription factors (TF) influence reprogramming? Isn’t it just the cells with the highest levels of TF expression that reprogram? So just blast them & it should work, right? 🤨Buckle up for our lab’s preprint & tour de force led by Nat Wang @nbwang22 ! (1/n)

[Nat B. Wang 🧬🧑🏻‍🔬🏳️‍🌈 @nbwang22]

Officially Nat Wang, PhD! ✅🎓

Grateful for all my incredible mentors and colleagues throughout this journey, especially my adviser @GallowayLabMIT

This marks the end of my education but still just the start of my research career. More exciting things coming 🔜🥳

[Katie Galloway @GallowayLabMIT]

First student hooded from the Galloway Lab! Congrats, Dr Wang! Excelsior!

@nbwang22 @MITChemE

[Stephanie Gaglione @Steph_Gaglione]

Thrilled to share our preprint! We introduce TCRAFT, a method to assemble tens of thousands of TCRs at <$1 each, unlocking high-throughput functional TCR screening from single-cell data.
🧵@kochinstitute @MEBirnbaum @MITChemE @MIT

https://t.co/zifGBP4p7k

[Katie Galloway @GallowayLabMIT]

For a long time we had wondered: what's more important for dictating the "reprogrammability" of cells? is it the cell state or is it the levels of transcription factor level? (1/n) :🧵
@nbwang22 @CellSystemsCP @MITChemE

[Katie Galloway @GallowayLabMIT]

Thrilled to join this group of scientist and grateful for the support of @MindPrize in our ambitious goal to change how therapies are delivered for repair of the CNS!

@MITChemE

[Albert Blanch Asensio @A_BlanchAsensio]

🚨Preprint alert!
Excited to share the latest work of my PhD on bioRxiv.
STRAIGHT-IN Dual allows the generation of homogenous genetically-modified hiPSC populations 1) in one week, 2) with ~100% efficiency, 3) in a markerless, near-scarless approach and 4) with two payloads!🧵⬇️

[Nat B. Wang 🧬🧑🏻‍🔬🏳️‍🌈 @nbwang22]

Awesome work from @A_BlanchAsensio !

Really excited to see possibilities for building precisely controlled circuits to guide iPSC fate programming. Super useful tool! 🧫🧬 (see thread)

Check preprint out for great examples on how to build effective constructs in iPSC

[Katie Galloway @GallowayLabMIT]

So you want to engineer your hiPSCs, but targeting DNA payloads requires multiple slow, inefficient steps for each construct. What if we could accomplish multi-site integration seamlessly?
🚨Introducing STRAIGHT-IN Dual! 🧵 (1/n)
Link: https://t.co/4BKAf4Or90

[Naveen Jain @uffdaALLberries]

1/ Thank you to @GallowayLabMIT for highlighting my recent paper (https://t.co/ASaWIF1RJL) with @arjunrajlab! Their work was a major inspiration for us and helped us develop some of our most interesting experiments on primed state dynamics.
https://t.co/4WPTa8HV89

[Pereira Lab @CellReproLab]

1/23 We are excited to announce that our most recent study “In vivo dendritic cell reprogramming for cancer immunotherapy” was published in @ScienceMagazine.
https://t.co/yPDqKmpGnn
Here is a summary of what we discovered!

[Alex Vlahos (he/him) @AlexVlahos]

I am excited to share that I will be starting as a tenure track Assistant Professor in @CoulterBME at @GeorgiaTech in the upcoming year. I am very grateful of the unwavering support of my postdoctoral mentor, @SynBioGaoLab , and my PhD supervisor, Michael Sefton.

[Katie Galloway @GallowayLabMIT]

Loss of gene expression can compromise cellular fitness. In the case of such haploinsufficiency, replacement of the gene supplementation could rescue cell health. But what if you need a very precise dosage? Try ComMAND! 🧵 (1/n)
Link: https://t.co/VhyQdYRvLG

[Albert Blanch Asensio @A_BlanchAsensio]

Extremely happy to see the protocol manuscript for the STRAIGHT-IN platform out online and featured as protocol of the week @NatureProtocols.

[Katherine Steinberg @kjsteinb]

Happy to share that my latest project with @GallantGroupMIT is out now in JES! Check it out if you're interested in how SEI composition influences Li metal anode performance. Proud to be a part of this special issue celebrating electrochemistry at CWRU. https://t.co/1GjvIjzurl

[Nicole DelRosso @nicole_delrosso]

🧬How do transcriptional cofactors recognize which genes to regulate?🧬 Check out our new preprint from @fordycelab @BintuLab that tackles this question and provides the first-ever quantitative map of disordered transcriptional protein interactions (1/9) https://t.co/5UV8IafaHM

[Albert Blanch Asensio @A_BlanchAsensio]

Yesterday I had the pleasure to present the improved version of STRAIGHT-IN (v3.0) at #mSBW2024 in Boston. I was also glad to see some old colleagues and to meet new ones as well! Stay tuned for more 🧬

[Samantha Morris @morris_lab]

CellTag-multi is finally out in @NatureBiotech https://t.co/d2row5Ploc. We didn't make the cover this time, but I love what we put together with @DrawImpacts! Here's our fun vision of CellTag cell barcoding and capture across single-cell RNA-seq and ATAC-seq @kjkjindal

[bioRxiv Synthetic Biology @bioRxiv_synbio]

Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells https://t.co/utPmrv5siC #biorxiv_synbio

[Katie Galloway @GallowayLabMIT]

on unimodal setpoints and how to get them from synthetic promoters...

tweetorial coming soon...

or see you next Thursday #SEED2024 in Atlanta...

Stay tuned for more control systems coming soon...

[Nat B. Wang 🧬🧑🏻‍🔬🏳️‍🌈 @nbwang22]

Today I have a #SEED2024 poster on how we were able to convert accessible donor cells (skin) to millions of high value cells (neurons)

With efficient conversion, there’s lots of exciting future directions now possible! Stop by to chat more :)

[Katie Galloway @GallowayLabMIT]

Today at #SEED2024 stop by poster #60 to hear Nat Wang @nbwang22 tell you about designing systems for high efficiency conversion to generate functional, engraftable neurons
#synbio
@MITChemE

https://t.co/xCedLA2mRh

[Katie Galloway @GallowayLabMIT]

Or stop by poster #28 to hear from Emma Peterman
@emmapeterman_

on her work on "Simultaneous measurement of protein and mRNA levels in single cells for genetic element characterization" in mammalian cells

#SEED2024 #synbio
@MITChemE

[Katie Galloway @GallowayLabMIT]

Today at #SEED2024 stop by poster #60 to hear Nat Wang @nbwang22 tell you about designing systems for high efficiency conversion to generate functional, engraftable neurons
#synbio
@MITChemE

https://t.co/xCedLA2mRh