James Halstead

A biologist trying to understand how cells make decisions, thinking one molecule at a time. Fascinated by all things RNA. Loves to share the excitement.

[James Halstead @thecellbrightly]

Thanks Rita! I do indeed love both DNA-PAINT and complaining about how slow it is! my super late nights on the scope may be over for now...

[Rita Strack @rita_strack]

If you love DNA-PAINT but also love to complain about how slow it can be...look no further than this paper from the Jungmann lab! And I already have independent confirmation that it works as described!

[James Halstead @thecellbrightly]

@IgorUlitsky @ACDbio Hi Igor, I'm going through some very similar issues myself (with pricing, nothing to do with tissue in FISH). did you solve this issue? I'm road testing a few options and will let you know...

[James Halstead @thecellbrightly]

@zenklusd @binwu1 @BeAScientist @sri1989vathsan @nliving93 only just read this now, but beautiful work.

[James Halstead @thecellbrightly]

Dear RNA twitter, I usually use Stellaris 48x20mer probes for smFISH but now need to target smaller RNA regions (>200nt). I guess bDNA technology is the tool. Any experience with RNAscope ViewRNA, others etc?... thanks
@jrRNAscientists @grnfluoresceblg @ilandavislab @arjunrajlab

[James Halstead @thecellbrightly]

as someone new to the Aus grant system I found this analysis particularly useful! recommended! @EMBLAustralia @UNSWScience @UNSWMedicine @SingMolSci @CentenaryInst

[Prof Danny Hatters @DannyHatters]

Excellent insight and thoughts on grant statistics from my very own post-doc @dezeraecox. Someone I can vouch is thoughtful and considered. A must read!

[James Halstead @thecellbrightly]

@dezeraecox @thomasrcox excellent stuff. thanks for doing this!

[James Halstead @thecellbrightly]

this could be the change so many of us ECRs have been hoping for? I'm feeling lucky...this could be big.

let's shake this publication game up like an etch-a-sketch!

@mleptin @embo @Co_Biologists @eLife @LSAjournal @Plos @JcellBiol @EMBOPress @SingMolSci @UNSWScience

[Review Commons @ReviewCommons]

Review Commons has "a strong group of community-minded academic journals joining this ambitious project" says @embo director @mleptin. We're thrilled to be working with @MBoCjournal, @Co_Biologists, @eLife, @LSAjournal, @PLOS, @JCellBiol, @EMBOPress https://t.co/X3qIK3HvNy

[Biology Open @BiologyOpen]

A new month means a new issue! On the cover in October - V5-tagged Apolipoprotein L9 stably expressed in B16F10 mouse melanoma cells. Find out what else is inside: https://t.co/bBaCooYEbN

[Ribosome Studio @RibosomeStudio]

Molecular Size Comparison

[James Halstead @thecellbrightly]

ooooh. fun.

[James Halstead @thecellbrightly]

the #NHMRC poured me big fat glass of #NFFCD today. here's to an even bigger drink of #neverletthemgetyoudown and good luck to all still hoping!

[James Halstead @thecellbrightly]

@pstansfeld @RobKlose @OxBiochNews @WarwickLifeSci congratulations! I never really worked with you phill, but have fond memories of failing to defend against you at biochem 5-a-side. good luck!

[Greg Jefferis @gsxej]

PhD opportunity in our group. Wide range of questions re neural circuits for learned and unlearned behaviour. Fully funded options for students from anywhere in the world, amazing scientific environment at @MRC_LMB and close collaboration with @flyconnectome @CamZoology. Pls RT!

[Rossjohn lab @RossjohnLab]

Now hiring: interested in working in the Rossjohn Lab? We have several postdoc positions available https://t.co/h56pTuLu1j

[James Halstead @thecellbrightly]

@Alf_Castello @castello_lab @bones_giles @ilandavislab awesome memories. @bones_giles was always very werewolfy in my opinion...

[James Halstead @thecellbrightly]

Dear Aus twitter, I'm looking for a dox-inducible Hela Cell line with a single integration site, such as HeLa-EM2-11ht, or HeLa Flp-In T-REx. Rather than fly one over from afar, does anyone have a spare vial here in Aus (or nearby)? will happily pay for courier! please RT! Thx

[UNSW Science @UNSWScience]

We're celebrating at #UNSWDiversityFest today with a special #WomenInSTEAM photo exhibition that portrays the humanness of our scientists and the capability of women, coordinated by @MelissaPappas10, @unswbees.

#equity #STEM #WomenInSTEM🚺

[James Halstead @thecellbrightly]

Two brilliant papers (one older, one new) from the Larson group that take us from single molecule quantitative RNA imaging, to a novel mechanism in cancer progression.

Gosh. It's almost as if basic biology is useful...

https://t.co/PF2GXa5xwM
https://t.co/75SCwwBTVN

[James Halstead @thecellbrightly]

Delighted and honoured to be part of this! Can't wait to speak about the future (imperfect!) of cell biology, and how it can help us in a future with limited pharmaceuticals... Bring on the #cellrevolution! @SingMolSci @UNSWMedicine

[Abolaji Amos @amosabolaji]

A warm welcome to @ilandavis in Nigeria as he graciously accepted @african_2019 invitation to the First African Society of #Drosophilsts Scientific Meeting @unibadan. @DrosLabUI @ICGEB @DrosAfrica