Thrilled to have our recent work from the @marsonlab published in @ScienceMagazine https://t.co/B24wWo2ERJ. This was the result of an awesome collaboration between myself and fellow Marson Lab postdoc, @R_Schmidt_ (1/12)
Excited to share that the Arce Lab is launching today at @arcinstitute !
We will explore the mechanisms controlling immune cell function using genomics and biochemistry in an effort to understand immune-mediated diseases. https://t.co/Ul7Bs7T2ti
Excited to share the latest from the @MarsonLab! An investigation of context-specific gene regulation in T cells, which led to dynamic mechanisms required to control T cell rest and activation.
Published today in @Nature thanks to the work of many!
https://t.co/eS0wpVeQQB
Excited to share a milestone in our forward genetics developments! Today in @Nature we report massive mutagenesis screening in T cells with base editors, increasing resolution from genes to nucleotides https://t.co/uIp3iGjrJ4
A @MarsonLab story with my dear friend @carlcward
1/10
I am very excited to share our paper on the interactions between #gdTcells and cancer cells, out today in @Nature! Using #CRISPR screens, we uncovered unexpected pathways in cancer cells regulating target molecules recognized by γδ T cells. @MarsonLab
1/2
https://t.co/wr66P63kO1
Happy to share the newest paper from our lab. Led by technician extraordinaire Aidan Tousley (soon to be an MD/PHD student!), this manuscript details our attempts to reimagine the structure of CARs to improve their specificity and efficacy. https://t.co/EKzcX4JaFP
Thrilled to share the first Eyquem lab paper! Today we report in @CellCellPress evolving the AAV6 capsid to enable efficient engineering of murine T cells. An exciting collab with @asokanduke and led by the brilliants @WilliamNyberg and Jon Ark
https://t.co/oYgEn7sBjF 🧵1/11
@kischober @JohnDoench @R_Schmidt_@KrisHaurum@MangusoLab We oft observe the inverse, where some selected cells don't have kd w CRISPRi. Exosome/endosome transfer of guides is an interesting thought. We certainly see a lot of "soup" guides in our T cell perturb-seq suggesting intact guides are released from dying cells or secreted (2/2)
@kischober @JohnDoench @R_Schmidt_@KrisHaurum@MangusoLab Interesting. My experience is mostly with puro selection on the sgRNA cassette, so I can't look at the negative population. My guess is that there is some low rate of recombination during lenti packaging where the GFP cassette is interrupted but the sgRNA cassette is intact (1/2)
I’m hiring a computational biologist at Genentech to analyze cutting-edge single-cell genomics and high-throughput screening data in an extremely collaborative environment focused on finding cures for disease. Please reach out to learn more.
https://t.co/RsMaiTo9O1
I'm very excited to share our review article https://t.co/9NDXE9ZpXl @NatRevImmunol where we discussed how IL-2 has been engineered since its discovery to improve its in vivo activity and T cell selectivity and drive therapeutic efficacy in the context of autoimmunity and cancer.
Thrilled to have our recent work from the @marsonlab published in @ScienceMagazine https://t.co/B24wWo2ERJ. This was the result of an awesome collaboration between myself and fellow Marson Lab postdoc, @R_Schmidt_ (1/12)