Nader

[ChloeXWang @ChloeXWang1]

1/7 First of all, big shoutout to co-authors on modeling (@MKarimzade, @neal_ravindra, @RexMa9, @HAOTIANCUI1, @LeeTaliq), huge appreciation to data generation (Lexi, @alerasool, Adam) and bioinformatics team (@_annhuang), and leadership for vision and direction (@BoWang87, @inCiChu)!
Preprint is now live on bioRxiv: https://t.co/31ei4v8huk

All models start from high-quality data.

[Mikko Taipale @mike_tilapia]

Our latest paper is out! In collaboration with @DrAnneCarpenter, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/ https://t.co/pQzVx0hOrC

[Mikko Taipale @mike_tilapia]

Our latest work on developing a potent and specific tool for activating or repressing endogenous splicing events is out! https://t.co/qHn6LEVsMC

[The Blencowe Lab @ U of T @Blencowe_Lab]

Graduate student Jack Li's work out in Molecular Cell now: By conducting a screen of 341 splicing factors, Jack identified RBM25 coupled to dCasRx as a superior tool to boost inclusion of endogenous exons /1

[Thomas Gonatopoulos-Pournatzis @TGonatopoulos]

Check out our CRISPR genome-scale exon perturbation study @MolecularCell. We identified exons influencing cell fitness and uncovered a novel mechanism by which #splicing affects #transcription. @Michael_Aregger @dlarun88 @meisheng_xiao @NCIResearchCtr @NIH
https://t.co/sZDNnmiJYD

[Mikko Taipale @mike_tilapia]

Our paper on functional discovery of protein degradation and stabilization effectors with proteome-scale induced proximity screens is out in its final version! 1/ https://t.co/vzNbJyR2rk https://t.co/5S9i7UG7JL

[Jacob Kimmel @jacobkimmel]

early winter @newlimit:

- >10X more reprogramming factor sets tested than ever before
- >2M cells profiled, 10TB+ of raw seq data
- +3 team members
- 2X speed up in reprogramming library build times
- +1 cell types on our Engine
- 1st rejuvenating hits

[Filip Nemcko @FilipNemcko]

📜 Happy to share our recent preprint featuring ORFtag - a versatile, easy and cost-effective method for probing protein function at a proteome scale. This work is the result of a collaborative effort by the @stark_lab, @AmeresSL, @EllingUlrich and @juliusbrennecke labs. 1/2

[Jacob Kimmel @jacobkimmel]

2023 @newlimit:

- 50X more reprogramming factor sets tested than the field before us
- 20X factor demultiplexing performance (reading), 7X factor delivery (writing)
- 2X team size
- +3 functional assays for immunology program
- +2 in silico model metrics > SOTA

blog ⬇️

[Induxion Therapeutics @induxiontx]

Induxion is looking for a Research Associate/Technician to support its platform activities - Located in Toronto. https://t.co/2oHlYEw8CA

[Jacob Kimmel @jacobkimmel]

early autumn @newlimit:

- 6X'd the # of reprogramming factors we've tested
- >4X'd signal-to-noise for our core pooled screening assay
- shortened feedback cycles by 40%
- improved in silico reprog models >30%

all covered in our latest blog

[Ibrahim Tannira @ibstigator]

Help me find my stolen bike! https://t.co/9RyXbRaA0y

[Mikko Taipale @mike_tilapia]

Our latest preprint is out! In a fun collaboration with @DrAnneCarpenter, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/8
https://t.co/Oebh0OaZHw

[Nader @alerasool]

@DonnellyCentre @UofT @mike_tilapia @MoGen_Grad Thank you so much @DonnellyCentre! & bug thanks to the glorious leader @mike_tilapia, who doesn’t just fight like a dairy farmer but is full of innovative ideas and is an amazing supervisor. Miss you all ❤️

[Andy Fraser @andy_utoronto]

Ever wondered if you could read 100s of metabolites and drugs in parallel using a @illumina or a @10X platform? But metabolites aren’t nucleic acids — how can that work? Check out our new preprint and read on for a thread:
https://t.co/0HLfwbz7Gy.

[NewLimit @newlimit]

We're excited to announce that we've raised our Series A from @_DimensionCap, @foundersfund, & @kleinerperkins

https://t.co/S33ESUenB6

[Josh Tycko @JoshTycko]

New preprint! 🗞️ We used pooled protein screens to develop compact human transcriptional effectors that work across diverse biological contexts to activate or silence gene expression

https://t.co/vwqDoUav0i

[Jacob Kimmel @jacobkimmel]

last month @newlimit:
- 1st reprogramming screens in primary cells
- Immunology team 3X'd
- 1st model-driven hypotheses

this month:

- improving screen efficiency: more exps/{$, time}
- multi-modal in silico reprogramming models

all this & more in our April update ⬇️

[Mikko Taipale @mike_tilapia]

Our paper on 14-3-3 proteins and their role in protein aggregation and phase separation is out! This paper represents the main PhD thesis work by my student Dmitri Segal. 1/ https://t.co/oCGBmHdrKS

[Jacob Kimmel @jacobkimmel]

last month @newlimit:

- largest partial reprogramming screen yet
- 50% more TFs to test
- first in silico reprogramming models

this month:

- screens in old donor cells
- library rebuild -- 10X manufacturing efficiency
- automating screen analysis to shorten DBTL loops